Recombinant Arabidopsis thaliana Ubiquitin-like protein ATG12A (ATG12A)

Code CSB-YP844608DOA
MSDS
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Source Yeast
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Code CSB-EP844608DOA
MSDS
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Source E.coli
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Code CSB-EP844608DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844608DOA
MSDS
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Source Baculovirus
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Code CSB-MP844608DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATG12A
Uniprot No.
Alternative Names
ATG12A; APG12A; At1g54210; F20D21.38Ubiquitin-like protein ATG12A; Autophagy-related protein 12a; APG12-like protein a; AtAPG12a
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-96
Target Protein Sequence
MATESSSPSS VRKVVVHLRA TGGAPILKQS KFKIPGTDKF AKVIDFLRRQ LHSDSLFVYV NSAFSPNPDE SVIDLYNNFG FDGKLVVNYA CSMAWG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. ATG12/ATG5 conjugate has an essential role in plant nutrient recycling.
Gene References into Functions
  1. The ATG12b locus is more important during basal autophagy while the ATG12a locus is more important during induced autophagy. PMID: 20136727
  2. In vitro reconstitution of plant Atg8 and Atg12 conjugation systems essential for autophagy PMID: 18039664
Subcellular Location
Cytoplasm.
Protein Families
ATG12 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT1G54210

STRING: 3702.AT1G54210.1

UniGene: At.43297

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