Recombinant Arabidopsis thaliana WD repeat-containing protein LWD2 (LWD2)

Code CSB-YP657131DOA
MSDS
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Source Yeast
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Code CSB-EP657131DOA
MSDS
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Source E.coli
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Code CSB-EP657131DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657131DOA
MSDS
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Source Baculovirus
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Code CSB-MP657131DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LWD2
Uniprot No.
Alternative Names
LWD2; AN11B; At3g26640; MLJ15.3; orf10WD repeat-containing protein LWD2; Protein ANTHOCYANIN 11-B; AtAN11-B; Protein LIGHT-REGULATED WD2; WD repeat-containing protein AN11-B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-346
Target Protein Sequence
MVTSSDQIQN GSEEQSKRSE IYTYEAPWQI YAMNWSIRRD KKYRLAITSL IEQYPNRVEI VQLDESNGEI RSDPNLCFEH PYPPTKTSFI PDKECQRPDL LATSSDFLRL WRISDDESRV ELKSCLSSDK NSEFSGPITS FDWNEAEPRR IGTSSIDTTC TIWDIEREVV DTQLIAHDKE VYDIAWGGVG VFASVSEDGS VRVFDLRDKE HSTIIYESGE PSTPLVRLSW NKQDPRYMAT VIMGSAKIVV LDIRFPALPV VELQRHQASV NAIAWAPHSS SHICSAGDDS QALIWDISSM GQHVEGGLDP ILAYTAGAEV EQLQWSSSQP DWVAIAFSNK LQILRV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Clock protein essential for the proper expression phase and period length of both the oscillator and output genes known to participate in photoperiod sensing. Required for the expression of APRR9, APRR7, and APRR5. Regulated by APRR9 and APRR7 at the transcriptional level, indicating the existence of a positive feedback loop within the circadian clock.
Gene References into Functions
  1. LWD1/2 controls the amplitude and period length but not the robustness of the Arabidopsis circadian rhythm under continuous light. PMID: 21357491
  2. LWD1 and LWD2 are WD-containing clock proteins that regulate photoperiodic flowering. [LWD2] PMID: 18676661
Database Links

KEGG: ath:AT3G26640

STRING: 3702.AT3G26640.1

UniGene: At.19781

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