Recombinant Arabidopsis thaliana 14-3-3-like protein GF14 upsilon (GRF5)

Code CSB-YP337159DOA
MSDS
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Source Yeast
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Code CSB-EP337159DOA
MSDS
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Source E.coli
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Code CSB-EP337159DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337159DOA
MSDS
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Source Baculovirus
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Code CSB-MP337159DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRF5
Uniprot No.
Alternative Names
GRF5; At5g16050; F1N13_19014-3-3-like protein GF14 upsilon; General regulatory factor 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-268
Target Protein Sequence
MSSDSSREEN VYLAKLAEQA ERYEEMVEFM EKVAKTVETE ELTVEERNLL SVAYKNVIGA RRASWRIISS IEQKEDSRGN SDHVSIIKDY RGKIETELSK ICDGILNLLE AHLIPAASLA ESKVFYLKMK GDYHRYLAEF KTGAERKEAA ESTLVAYKSA QDIALADLAP THPIRLGLAL NFSVFYYEIL NSSDRACSLA KQAFDEAISE LDTLGEESYK DSTLIMQLLR DNLTLWTSDL NDEAGDDIKE APKEVQKVDE QAQPPPSQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. May be involved in cell cycle regulation by binding to soluble EDE1 and sequestering it in an inactive form during the early stages of mitosis.
Gene References into Functions
  1. GRF5 stimulates chloroplast division, resulting in a higher chloroplast number per cell with a concomitant increase in chlorophyll levels. PMID: 25604530
  2. 14-3-3 upsilon most strongly associates with EDE1 in its free form, but also weakly when EDE1 is bound to microtubules. [14-3-3 upsilon] PMID: 21558460
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
14-3-3 family
Database Links

KEGG: ath:AT5G16050

STRING: 3702.AT5G16050.1

UniGene: At.25563

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