Recombinant Arabidopsis thaliana Cyclin-D3-1 (CYCD3-1)

Code CSB-YP337197DOA
MSDS
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Source Yeast
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Code CSB-EP337197DOA
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Source E.coli
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Code CSB-EP337197DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337197DOA
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Source Baculovirus
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Code CSB-MP337197DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYCD3-1
Uniprot No.
Alternative Names
CYCD3-1; CYCD3; At4g34160; F28A23.80Cyclin-D3-1; Cyclin-delta-3; Cyclin-d3; G1/S-specific cyclin-D3-1; CycD3;1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-376
Target Protein Sequence
MAIRKEEESR EEQSNSFLLD ALYCEEEKWD DEGEEVEENS SLSSSSSPFV VLQQDLFWED EDLVTLFSKE EEQGLSCLDD VYLSTDRKEA VGWILRVNAH YGFSTLAAVL AITYLDKFIC SYSLQRDKPW MLQLVSVACL SLAAKVEETQ VPLLLDFQVE ETKYVFEAKT IQRMELLILS TLEWKMHLIT PISFVDHIIR RLGLKNNAHW DFLNKCHRLL LSVISDSRFV GYLPSVVAAA TMMRIIEQVD PFDPLSYQTN LLGVLNLTKE KVKTCYDLIL QLPVDRIGLQ IQIQSSKKRK SHDSSSSLNS PSCVIDANPF NSDESSNDSW SASSCNPPTS SSSPQQQPPL KKMRGAEENE KKKPILHLPW AIVATP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the control of the cell cycle at the G1/S (start) transition. Activates the G1/S phase transition in response to cytokinin hormone signal, but declines in response to sucrose starvation leading to G1 arrest. Involved in the induction of mitotic cell division. Plays an important role in the switch from cell proliferation to the final stages of differentiation during plant development. May not be involved in the activation of cell cycle in the root apical meristem (RAM) in the early phase of seed germination. Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division.
Gene References into Functions
  1. ANT does not regulate CYCD3;1 during petal growth. PMID: 25948704
  2. The direct target of both the bacterial cytokinins and plant putrescine was the expression of D3-type cyclins. PMID: 21459976
  3. CYCD3;1 dominantly drives the G1/S transition, and in sucrose-depleted cells the decline in CYCD3;1 levels leads to G1 arrest, which is overcome by ectopic CYCD3;1 expression. PMID: 16517759
  4. A combined action of ANT and CYCD3;1 promotes cell proliferation in leaf primordia and increases leaf size. PMID: 19508432
  5. Overlap between CYCD3;1 and E2Fa modulated genes substantiates their action in a common pathway with a key role in controlling the G1/S transition, with additional targets for CYCD3;1 in chromatin modification. PMID: 19662336

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Protein Families
Cyclin family, Cyclin D subfamily
Tissue Specificity
Highly expressed in roots and at lower levels in leaves and flowers. Expressed in vegetative shoot meristem and inflorescence.
Database Links

KEGG: ath:AT4G34160

STRING: 3702.AT4G34160.1

UniGene: At.22721

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