Recombinant Arabidopsis thaliana Serine/threonine-protein kinase AtPK1/AtPK6 (ATPK1)

Code CSB-YP337226DOA
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Source Yeast
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Code CSB-EP337226DOA
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Source E.coli
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Code CSB-EP337226DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337226DOA
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Source Baculovirus
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Code CSB-MP337226DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATPK1
Uniprot No.
Alternative Names
ATPK1; ATPK6; S6K1; At3g08730; F17O14.20Serine/threonine-protein kinase AtPK1/AtPK6; EC 2.7.11.1; Ribosomal-protein S6 kinase homolog 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-465
Target Protein Sequence
MVSSQRPVPN KIQKQQYLSI SPSNSVLKDD VELEFSDVFG PLPEEANDIA YDEPAVVYSR SHSLVGPCSL DSHSLKLTKL TLLETEDSID LVECLEGESL KENDDFSGND DSDNEKALEG DLVKVSGVVG IDDFEVMKVV GKGAFGKVYQ VRKKETSEIY AMKVMRKDHI MEKNHAEYMK AERDILTKID HPFIVQLKYS FQTKYRLYLV LDFINGGHLF FQLYHQGLFR EDLARVYTAE IVSAVSHLHE KGIMHRDLKP ENILMDTDGH VMLTDFGLAK EFEENTRSNS MCGTTEYMAP EIVRGKGHDK AADWWSVGIL LYEMLTGKPP FLGSKGKIQQ KIVKDKIKLP QFLSNEAHAI LKGLLQKEPE RRLGSGLSGA EEIKQHKWFK GINWKKLEAR EVMPSFKPEV SGRQCIANFD KCWTDMSVLD SPASSPSSDP KANPFTNFTY VRPPPSFLHQ STTTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway. Mediates the phosphorylation of MRFs (e.g. MRF1).
Gene References into Functions
  1. A 12-amino acid core element in S6 kinase 1 (AtS6K1) was identified as an essential interacting region with Raptor1 protein. PMID: 28285138
  2. identified a 44-amino acid region at the N-terminal end of ribosomal S6 kinase 1 (S6K1) specifically interacted with Raptor1, indicating that this region may contain a functional equivalent of TOS (TOR-Signaling) motif present in mammalian TOR substrates PMID: 26920057
  3. S6K1 interacts with retinoblastoma related protein (RBR)1 via its N-terminal RBR binding motif, promotes its nuclear localization and consequent RBR-dependent repression of cell cycle genes through transcription factor E2FB. PMID: 23531690
  4. Authors propose that TOR functions in polysomes to maintain the active S6K1 (and thus eIF3h) phosphorylation status that is critical for translation reinitiation. PMID: 23524850
  5. The authors found that S6K1 associates with the Retinoblastoma-related 1 (RBR1)-E2FB complex and this is partly mediated by its N-terminal LVxCxE motif. PMID: 20683442
  6. Arabidopsis RAPTOR1 (a TOR regulatory protein) interacts with the HEAT repeats of TOR and that RAPTOR1 regulates the activity of S6 kinase (S6K) in response to osmotic stress [S6K1] PMID: 16377759

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
Tissue Specificity
Expressed in all tissues.
Database Links

KEGG: ath:AT3G08730

STRING: 3702.AT3G08730.1

UniGene: At.23700

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