Recombinant Arabidopsis thaliana Transcription factor TGA2 (TGA2)

Code CSB-YP337365DOA
MSDS
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Source Yeast
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Code CSB-EP337365DOA
MSDS
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Source E.coli
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Code CSB-EP337365DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337365DOA
MSDS
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Source Baculovirus
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Code CSB-MP337365DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TGA2
Uniprot No.
Alternative Names
TGA2; BZIP20; HBP1B; At5g06950; MOJ9.12; Transcription factor TGA2; HBP-1b homolog; AHBP-1b; bZIP transcription factor 20; AtbZIP20
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-330
Target Protein Sequence
MADTSPRTDV STDDDTDHPD LGSEGALVNT AASDSSDRSK GKMDQKTLRR LAQNREAARK SRLRKKAYVQ QLENSRLKLT QLEQELQRAR QQGVFISGTG DQAHSTGGNG ALAFDAEHSR WLEEKNKQMN ELRSALNAHA GDSELRIIVD GVMAHYEELF RIKSNAAKND VFHLLSGMWK TPAERCFLWL GGFRSSELLK LLANQLEPMT ERQLMGINNL QQTSQQAEDA LSQGMESLQQ SLADTLSSGT LGSSSSGNVA SYMGQMAMAM GKLGTLEGFI RQADNLRLQT LQQMIRVLTT RQSARALLAI HDYFSRLRAL SSLWLARPRE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Required to induce the systemic acquired resistance (SAR) via the regulation of pathogenesis-related genes expression. Binding to the as-1 element of PR-1 promoter is salicylic acid-inducible and mediated by NPR1. Could also bind to the C-boxes (5'-ATGACGTCAT-3') with high affinity.
Gene References into Functions
  1. Our study showed that TGA1 and TGA4 regulate Pip and SA biosynthesis by modulating the expression of SARD1 and CBP60g. PMID: 28898429
  2. AtNPR1, AtGA2, and AtPR-5, encoding specific components involved in salicylic acid regulation, synthesis, and signaling, are overexpressed in soybean roots, resistance to soybean cyst nematode is enhanced. PMID: 24739302
  3. TGA2-binding motif is directly involved in the modulation of salicylic acid-induced NIMIN-1 expression in Arabidopsis. PMID: 20198573
  4. We confirm that the NPR1 BTB/POZ domain interacts with and negates the molecular function of the TGA2 repression domain by excluding TGA2 oligomers from cognate DNA. PMID: 19915088
  5. NPR1 may transiently interact with the DNA unbound fraction of TGA2 to promote its recruitment to an active form on cognate target promoters. PMID: 18571510

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Subcellular Location
Nucleus.
Protein Families
BZIP family
Tissue Specificity
Expressed in the whole plant.
Database Links

KEGG: ath:AT5G06950

STRING: 3702.AT5G06950.1

UniGene: At.23884

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