Recombinant Arabidopsis thaliana Trichome differentiation protein GL1 (GL1)

Code CSB-YP340923DOA
MSDS
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Source Yeast
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Code CSB-EP340923DOA
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Source E.coli
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Code CSB-EP340923DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340923DOA
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Source Baculovirus
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Code CSB-MP340923DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GL1
Uniprot No.
Alternative Names
GL1; MYB0; At3g27920; K16N12.17; Trichome differentiation protein GL1; Myb-related protein 0; AtMYB0; Protein GLABRA 1; Protein GLABROUS 1; R2R3-MYB transcription factor GL1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-228
Target Protein Sequence
MRIRRRDEKE NQEYKKGLWT VEEDNILMDY VLNHGTGQWN RIVRKTGLKR CGKSCRLRWM NYLSPNVNKG NFTEQEEDLI IRLHKLLGNR WSLIAKRVPG RTDNQVKNYW NTHLSKKLVG DYSSAVKTTG EDDDSPPSLF ITAATPSSCH HQQENIYENI AKSFNGVVSA SYEDKPKQEL AQKDVLMATT NDPSHYYGNN ALWVHDDDFE LSSLVMMNFA SGDVEYCL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in leaves. Together with TTG1 and GL3, promotes trichome formation and endoreplication. Regulates the production of a signal that induces hair (trichome) precursor cells on leaf primordia to differentiate. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes.
Gene References into Functions
  1. These results suggested that the Serine 92 in the conserved [D/E]Lx2 [R/K]x3Lx6Lx3R amino acid signature of GL1 is not essential for the interaction of GL1 and GL3, but may play a role in the binding of GL1 to the promoters of its target genes. PMID: 26667588
  2. GL1 represses the activation of the TRY promoter by GL3 and TTG1, and TTG1 suppresses the activation of the CPC promoter by GL1 and GL3. PMID: 25926482
  3. Protein interaction analysis revealed that MYB82 physically interacts with GLABRA3. In addition, MYB82 and GL1 can form homodimers and heterodimers at R2R3-MYB domains, which may explain why their overexpression reduces trichome numbers. PMID: 24803498
  4. AtMYC1 protein is predominantly localised in the cytoplasm and can relocate GL1 from the nucleus into the cytoplasm. PMID: 23900543
  5. GL1 has qualitative and likely quantitative effects on trichome density in natural populations of Arabidopsis thaliana. PMID: 22625421
  6. Impaired fatty acid/lipid flux is associated with a nonallelic GLABRA1 mutation which causes abnormal cuticle formation and impaired plant immunity. PMID: 20699396
  7. TRICHOMELESS1 can be recruited to the cis-acting regulatory elements of GLABRA1 PMID: 17933793
  8. Data show that GL1 associates with TRANSPARENT TESTA GLABRA1 in vivo, forming a complex. PMID: 18434419
  9. DNA binding of GL1 and GL3 via their own DNA-binding domains to the promoter of GL2 is probably required for the activation of GL2 expression. PMID: 18948276
  10. 20 novel genes in the regulatory network involved in the trichome formation are the direct targets of GL3/GL1. PMID: 19247443

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Subcellular Location
Nucleus. Note=Detected in trichome nucleus.
Tissue Specificity
Expressed in leaves, stems and flowers. Expressed in trichome cells and in leaf primordia.
Database Links

KEGG: ath:AT3G27920

STRING: 3702.AT3G27920.1

UniGene: At.42881

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