Recombinant Archaeoglobus fulgidus Probable DNA primase small subunit (priA)

Code CSB-YP519878DOC
MSDS
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Source Yeast
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Code CSB-EP519878DOC
MSDS
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Source E.coli
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Code CSB-EP519878DOC-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519878DOC
MSDS
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Source Baculovirus
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Code CSB-MP519878DOC
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
priS
Uniprot No.
Alternative Names
priS; priA; AF_0742; DNA primase small subunit PriS; EC 2.7.7.-
Species
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Expression Region
1-335
Target Protein Sequence
MLTKLFLKKK FEEYYSKNEV ELPRKFKNRE FAFVPLELLP DFVMHRHISF RSETDFRAYI LSNVPAHIYF SSAYYERPAE DKMENKGWLG ADLIFDIDAD HLPVKAQSFE KALEMAKREI KKLTAVLRAD FGIRDMKIYF SGGRGYHVHV HDEEFLSLGS AERREIVDYL RLNSPKIVVE DRFANSNAAK RVLNYLRKKL EEDERLTSKL KIKPADLKKE KLTKKVIRAV EKFDYSALSI YIDAPVTADV KRLIRLPGSL HGKTGLRVTE VEDIESFNPL KDALAFGDEA VVVKVARKLN LSIGDFSGKI YPGRVKLPEY AAVFLICRGD ASYDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.
Protein Families
Eukaryotic-type primase small subunit family
Database Links

KEGG: afu:AF_0742

STRING: 224325.AF0742

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