Recombinant Autographa californica nuclear polyhedrosis virus FAD-linked sulfhydryl oxidase (P33)

Code CSB-YP334852ARA
MSDS
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Source Yeast
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Code CSB-EP334852ARA
MSDS
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Source E.coli
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Code CSB-EP334852ARA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334852ARA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP334852ARA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
P33
Uniprot No.
Alternative Names
P33; ORF92FAD-linked sulfhydryl oxidase; p33; EC 1.8.3.2
Species
Autographa californica nuclear polyhedrosis virus (AcMNPV)
Expression Region
1-259
Target Protein Sequence
MIPLTPLFSR YKDSYLLYSF RLIDLLRASK STHLTKLLSS QATYLYHFAC LMKYKDIQKY EVQQLIEWAI NASPDMDLQQ FRIEFMDKTT ELNLRSCQPK SFTYTFTTIW DTMHFLSLII DDMVYTRDKS SLDFVMQQLK TMKVLFYNVF FILQCAMCRD HYMNVKGFII YHIELIEIAL DKEKYGTDIT FVDSYQQETA GADVAVVSNN MLMKNLMAYV SMTFHNHIND YKWIQRNKKP PAHYERMTWG EYKKLLNLQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functional FAD-linked sulfhydryl oxidase that is required for infectious budded virion (BV) production and for the formation of enveloped occluded virion (ODV).
Gene References into Functions
  1. results showed that the three conserved regions, i.e., the active site, the dimer interface, and the R127-E183 salt bridge, are key regions of P33 and play significant roles in virus morphogenesis and oral infectivity PMID: 28904203
  2. The Trichoplusia ni single nucleopolyhedrovirus tn79 gene is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the ac92 gene. PMID: 25010286
  3. Thus, although Ac92 binds to Spodoptera frugiperda P53 during Autographa californica M nucleopolyhedrovirus replication and oxidizes SfP53 in vitro, authors could not detect any effects of this interaction on virus replication. PMID: 24210115
  4. This novel assembly comprises a dimer of pseudodimers with a striking 40-degree kink in the interface helix between subunits. PMID: 21752922
  5. AC92 plays a key role in virion morphogenesis and is essential for the successful infection. PMID: 20965540

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Subcellular Location
Host cytoplasm. Host nucleus.
Protein Families
Baculoviridae p33 family
Database Links

KEGG: vg:1403925

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