Recombinant Autographa californica nuclear polyhedrosis virus Protein p74(P74) ,partial

Code CSB-YP322818ARA
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Source Yeast
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Code CSB-EP322818ARA
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Source E.coli
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Code CSB-EP322818ARA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322818ARA
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Source Baculovirus
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Code CSB-MP322818ARA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names P74
Uniprot No. P15963
Alternative Names P74; Per os infectivity factor 0; PIF0
Species Autographa californica nuclear polyhedrosis virus (AcMNPV)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Plays an essential role for oral infectivity of occlusion-derived virus (ODV) and functions also in host midgut attachment and fusion by binding to a specific host receptor.
Gene References into Functions
  1. P74 contains two transmembrane domains in its hydrophobic C terminus which play a role in transmembrane anchoring, and two conserved domains which are involved in P74 function.[review] PMID: 29996043
  2. The authors propose that under in vivo infection conditions, P74 undergoes two sequential cleavage events, the first one being performed by a virus-associated host alkaline protease and the second carried out by trypsin in the host midgut. PMID: 21849453
  3. The authors propose that PIF1, PIF2, PIF3, and P74 form an evolutionarily conserved complex on the occlusion-derived virus surface, which has an essential function in the initial stages of baculovirus oral infection. PMID: 20610731
  4. A soluble form of P74 can act as a per os infectivity factor to the Autographa californica multiple nucleopolyhedrovirus. PMID: 20007360
  5. Trypsin activates per os infectivity of baculovirus ODC by cleaving p74 at R195, R196 and R199 sites. PMID: 18796706

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Subcellular Location Secreted, Virion membrane, Multi-pass membrane protein
Protein Families Baculoviridae p74 family
Database Links

KEGG: vg:1403971

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