Recombinant BK polyomavirus Large T antigen, partial

Code CSB-YP360947BGY
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP360947BGY
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP360947BGY
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Large T antigen; LT; LT-AG; EC 3.6.4.-
Species
BK polyomavirus (BKPyV) (Human polyomavirus 1)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

What is the difference between CSB-MP360953BGY and CSB-MP360947BGY. Do they target different regions of the BK Large T antigen? Also, what is the cost of CSB-MP360953BGY for purchase?

A:
Thanks for your inquiry. Regarding your requests, here are the detailed information.
1.Recombinant BK polyomavirus Major capsid protein VP1
CSB-YP360953BGY >> Yeast N-terminal 6xHis-tagged
CSB-EP360953BGY >> E.coli N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged In Stock.
CSB-EP360953BGYb1 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged In Stock.
CSB-BP360953BGY >> Baculovirus N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
CSB-MP360953BGY >> Mammalian cell N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Expression region: 1-362aa; Full length
Sequence:

MAPTKRKGECPGAAPKKPKEPVQVPKLLIKGGVEVLEVKTGVDAITEVECFLNPEMGDPDENLRGFSLKLSAENDFSSDSPERKMLPCYSTARIPLPNLNEDLTCGNLLMWEAVTVQTEVIGITSMLNLHAGSQKVHEHGGGKPIQGSNFHFFAVGGEPLEMQGVLMNYRSKYPDGTITPKNPTAQSQVMNTDHKAYLDKNNAYPVECWVPDPSRNENARYFGTFTGGENVPPVLHVTNTATTVLLDEQGVGPLCKADSLYVSAADICGLFTNSSGTQQWRGLARYFKIRLRKRSVKNPYPISFLLSDLINRRTQRVDGQPMYGMESQVEEVRVFDGTERLPGDPDMIRYIDKQGQLQTKML


2-1.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 1-133aa; Fragment at N-terminus
Sequence:

MDKVLNREESMELMDLLGLERAAWGNLPLMRKAYLRKCKEFHPDKGGDEDKMKRMNTLYKKMEQDVKVAHQPDFGTWSSSEVPTYGTEEWESWWSSFNEKWDEDLFCHEDMFASDEEATADSQHSTPPKKKRK
2-2.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY1 >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY1 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY1 >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY1 >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 127 - 261aa; Partial protein
Sequence:

PPKKKRKVEDPKDFPSDLHQFLSQAVFSNRTLACFAVYTTKEKAQILYKKLMEKYSVTFISRHMCAGHNIIFFLTPHRHRVSAINNFCQKLCTFSFLICKGVNKEYLLYSALTRDPYHTIEESIQGGLKEHDFSP


2-3.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY2 >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY2 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY2 >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY2 >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 262 - 695aa; Fragment at C-terminus
Sequence:

EEPEETKQVSWKLITEYAVETKCEDVFLLLGMYLEFQYNVEECKKCQKKDQPYHFKYHEKHFANAIIFAESKNQKSICQQAVDTVLAKKRVDTLHMTREEMLTERFNHILDKMDLIFGAHGNAVLEQYMAGVAWLHCLLPKMDSVIFDFLHCIVFNVPKRRYWLFKGPIDSGKTTLAAGLLDLCGGKALNVNLPMERLTFELGVAIDQYMVVFEDVKGTGAESKDLPSGHGINNLDSLRDYLDGSVKVNLEKKHLNKRTQIFPPGLVTMNEYPVPKTLQARFVRQIDFRPKIYLRKSLQNSEFLLEKRILQSGMTLLLLLIWFRPVADFATDIQSRIVEWKERLDSEISMYTFSRMKYNICMGKCILDITREEDSETEDSGHGSSTESQSQCSSQVSDTSAPAEDSQRSDPHSQELHLCKGFQCFKRPKTPPPK

Target Background

Function
Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription.
Subcellular Location
Host nucleus.
Database Links

KEGG: vg:29031009

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1