Recombinant Ba (E.coli -k12) Flagellin protein

Code CSB-YP300968ENV
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Source Yeast
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Code CSB-EP300968ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP300968ENV
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Source Baculovirus
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Code CSB-MP300968ENV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fliC
Uniprot No.
Research Area
Others
Alternative Names
fliC; flaF; hag; b1923; JW1908Flagellin
Species
Escherichia coli (strain K12)
Expression Region
2-498aa
Target Protein Sequence
AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Gene References into Functions
  1. Studies indicate that toll-like receptor 5 (TLR5) specifically interacts with flagellin, and is useful as an analytical recognition element for low concentrations of flagellin. PMID: 24881544
  2. Flagellin subunit FliC function as adhesins to piglet epithelial cells invasion. PMID: 23746569
  3. Results showed a reproducible and clear cut classification with molecular polymorphism of gene rfb cluster and flagella gene fliC. PMID: 19656466
  4. Flagellin in E. coli Nissle 1917 is the major stimulatory factor of human beta-defensin. PMID: 17283097
  5. Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented PMID: 17702465
  6. Flagellin expression by uropathogenic Escherichia coli coincides with ascension of the ureters and colonization of the kidney. PMID: 17925449
  7. This study demonstrated that infection of intestinal epithelial cells by A/E pathogens can trigger an array of proinflammatory responses from epithelial cells through both FliC-dependent and -independent pathways. PMID: 18227166
  8. Insertion of Cys-derived thiol residues in the E. coli FliC protein increased Au(III) sorption and reduction onto the surface of the flagellar filament and resulted in the production of stabilized Au(0) nanoparticles of approximately 20-50 nm diameter. PMID: 18819156
  9. Binding of a fliC(H7) mutant O157 strain to rectal epithelium was significantly reduced as was binding of the flagellated wild-type strain following incubation with H7-specific antibodies. PMID: 19016776
  10. Increased IL-8 levels mainly depended on flagellin expression as fliC-negative EPEC did not elicit a significant IL-8 response despite increased mucosal colonization compared with wild-type EPEC. PMID: 19134113
  11. FliC is secreted by the LEE-encoded type III secretion system and stimulates TLR5 signalling but does not confer motility. PMID: 19200386

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Subcellular Location
Secreted. Bacterial flagellum.
Protein Families
Bacterial flagellin family
Database Links

KEGG: ecj:JW1908

STRING: 316407.1736591

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