Recombinant Bacillus halodurans Alpha-N-arabinofuranosidase (abfA)

Code CSB-YP888515BQS
MSDS
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Source Yeast
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Code CSB-EP888515BQS
MSDS
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Source E.coli
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Code CSB-EP888515BQS-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888515BQS
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP888515BQS
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
abfA
Uniprot No.
Alternative Names
abfA; BH1861; Intracellular exo-alpha-(1->5)-L-arabinofuranosidase; ABF; EC 3.2.1.55; Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase; Arabinosidase
Species
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Expression Region
1-500
Target Protein Sequence
MTLTATMVVD KSFKIGEIDK RIYGSFIEHL GRAVYEGIYE PGHPDGDEQG FRKDVIRLVQ ELQVPLVRYP GGNFVSGYNW EDGVGPVSER PKRLDLAWRT TETNEIGTNE FVDWAKKVGA EVNMAVNLGS RGVDAARNLV EYCNHPSGSY WSDLRISHGY KDPHNIKTWC LGNEMDGPWQ IGQKTAEEYG RVAAEAGKVM KLVDPSIELV ACGSSNSKMA TFADWEATVL DHTYDYVDYI SLHTYYGNRD DDLANYLAQS MDMDEFIRSV IAIADYVKAK KRSKKTIHLS FDEWNVWFHS NEADRQITPW SVAPPLLEDI YTFEDALLVG SMLITLLKHA DRVKIACLAQ LVNVIAPIMT EKGGPAWKQT IFYPYMHASV YGRGVALQAQ ISSPKYDSKD FTDVPYLDAA VVHLEEAEEV TIFAVNKHQT ESLNLQCDMR SFEGYHVLEH IVLEHENMKA TNQGREQVTP HHNGDSAIDQ GRLTANLAKL SWNVIRLGKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (branched and debranched arabinans).
Subcellular Location
Cytoplasm.
Protein Families
Glycosyl hydrolase 51 family
Database Links

KEGG: bha:BH1861

STRING: 272558.BH1861

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