Recombinant Bacillus subtilis MreB-like protein (mbl)

Code CSB-YP334611BRJ
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Source Yeast
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Code CSB-EP334611BRJ
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Source E.coli
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Code CSB-EP334611BRJ-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334611BRJ
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Source Baculovirus
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Code CSB-MP334611BRJ
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mbl
Uniprot No.
Alternative Names
mbl; BSU36410Cell shape-determining protein Mbl; Actin-like Mbl protein; Rod shape-determining protein Mbl
Species
Bacillus subtilis (strain 168)
Expression Region
1-333
Target Protein Sequence
MFARDIGIDL GTANVLIHVK GKGIVLNEPS VVALDKNSGK VLAVGEEARR MVGRTPGNIV AIRPLKDGVI ADFEVTEAML KHFINKLNVK GLFSKPRMLI CCPTNITSVE QKAIKEAAEK SGGKHVYLEE EPKVAAIGAG MEIFQPSGNM VVDIGGGTTD IAVISMGDIV TSSSIKMAGD KFDMEILNYI KREYKLLIGE RTAEDIKIKV ATVFPDARHE EISIRGRDMV SGLPRTITVN SKEVEEALRE SVAVIVQAAK QVLERTPPEL SADIIDRGVI ITGGGALLNG LDQLLAEELK VPVLVAENPM DCVAIGTGVM LDNMDKLPKR KLS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and Mbl localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature. Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on Mbl polymerization. Organizes peptidoglycan synthesis in the lateral cell wall. Also required for proper chromosome segregation.
Gene References into Functions
  1. findings show that the mbl mutant phenotype is Mg2+ dependent, and a screen for suppressor mutations revealed that activation of the sigma I regulon overcomes the requirement for Mg2+ PMID: 19114499
Subcellular Location
Cytoplasm.
Protein Families
FtsA/MreB family
Database Links
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