Recombinant Bacillus subtilis Protein smf (smf)

Code CSB-YP340160BRJ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP340160BRJ
MSDS
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Source E.coli
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Code CSB-EP340160BRJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340160BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP340160BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dprA
Uniprot No.
Alternative Names
dprA; smf; BSU16110; DNA processing protein DprA; Protein Smf
Species
Bacillus subtilis (strain 168)
Expression Region
1-297
Target Protein Sequence
MDQAAVCLTI CRINQLLSPS LLLKWWKADP SMSLTSPVLQ TVTRDQIKAA ALKNEIEQFY PKLPRVLAAY REQGINTIPI SSKQYPFWLK SIYDPPAVLF AKGDMTLLSK GRKIGIVGTR NPTAYGKQVV NHLTKEICRK GWVIVSGLAS GIDGMSHAAS IKAKGRTIGV IAGGFQHIYP RENLQLADHM AKHHILLSEH PPETKPQKWH FPMRNRIISG LSEGVIVVQG KEKSGSLITA YQALEQGREV FAVPGSLFDP YAGGPIKLIQ QGAKAIWSAE DIFEELPERN VQYTEPF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein that helps load RecA onto ssDNA during transformation. Binds cooperatively to circular ssDNA, is able to bridge different segments of DNA. Favors the loading of RecA onto SsbA- or SsbB-coated ssDNA and formation of RecA-DNA filaments. RecA-ATP cannot catalyze homologous DNA strand exchange; SsbA and DprA activate strand exchange by RecA-ATP.
Gene References into Functions
  1. DprA and Smf bind cooperatively to ssDNA and that these proteins both self-interact and interact with RecA. We demonstrate that DprA-RecA-ssDNA filaments are produced and that these filaments catalyze the homology-dependent formation of joint molecules. PMID: 17803906
Subcellular Location
Cytoplasm.
Protein Families
DprA/Smf family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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