Recombinant Bothrops jararacussu Phospholipase A2 bothropstoxin-2

Code CSB-YP338346BUX
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Source Yeast
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Code CSB-EP338346BUX
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Source E.coli
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Code CSB-EP338346BUX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338346BUX
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Source Baculovirus
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Code CSB-MP338346BUX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Basic phospholipase A2 homolog bothropstoxin-2; svPLA2 homolog; BJU-PLA2; BJUPLA2; BOJU-II; Bothropstoxin-II; Bothropstoxin II; BthTX-II; BtxtxII; Myotoxic phospholipase A2
Species
Bothrops jararacussu (Jararacussu)
Expression Region
17-138
Target Protein Sequence
DLWQ FGQMILKETG KLPFPYYTTY GCYCGWGGQG QPKDATDRCC FVHDCCYGKL TNCKPKTDRY SYSRENGVII CGEGTPCEKQ ICECDKAAAV CFRENLRTYK KRYMAYPDVL CKKPAEKC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Snake venom phospholipase A2 (PLA2) that shows low enzymatic activity even tough it conserves the catalytic residues. Shows a strong myotoxic activity and induces indirect hemolysis, anticoagulant properties, and cytotoxic activities. In vivo, it induces muscle necrosis, accompanied by polymorphonuclear cell infiltration, and edema in the mouse paw. It exerts its function even in the absence of extracellular calcium, indicating it is not a calcium-dependent enzyme. A model of myotoxic mechanism has been proposed: an apo Lys49-PLA2 is activated by the entrance of a hydrophobic molecule (e.g. fatty acid) at the hydrophobic channel of the protein leading to a reorientation of a monomer. This reorientation causes a transition between 'inactive' to 'active' states, causing alignment of C-terminal and membrane-docking sites (MDoS) side-by-side and putting the membrane-disruption sites (MDiS) in the same plane, exposed to solvent and in a symmetric position for both monomers. The MDoS region stabilizes the toxin on membrane by the interaction of charged residues with phospholipid head groups. Subsequently, the MDiS region destabilizes the membrane with penetration of hydrophobic residues. This insertion causes a disorganization of the membrane, allowing an uncontrolled influx of ions (i.e. calcium and sodium), and eventually triggering irreversible intracellular alterations and cell death.
Subcellular Location
Secreted.
Protein Families
Phospholipase A2 family, Group II subfamily, D49 sub-subfamily
Tissue Specificity
Expressed by the venom gland.
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