Recombinant Bovine Glycine N-acyltransferase (GLYAT)

Code CSB-YP643453BO
MSDS
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Source Yeast
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Code CSB-EP643453BO
MSDS
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Source E.coli
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Code CSB-EP643453BO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP643453BO
MSDS
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Source Baculovirus
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Code CSB-MP643453BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GLYAT
Uniprot No.
Alternative Names
GLYATGlycine N-acyltransferase; EC 2.3.1.13; Acyl-CoA:glycine N-acyltransferase; AAc; Aralkyl acyl-CoA N-acyltransferase; Aralkyl acyl-CoA:amino acid N-acyltransferase; Benzoyl-coenzyme A:glycine N-acyltransferase; Glycine N-benzoyltransferase; EC 2.3.1.71
Species
Bos taurus (Bovine)
Expression Region
1-295
Target Protein Sequence
MFLLQGAQML QMLEKSLRKS LPMSLKVYGT VMHMNHGNPF NLKALVDKWP DFQTVVIRPQ EQDMKDDLDH YTNTYHVYSE DLKNCQEFLD LPEVINWKQH LQIQSTQSSL NEVIQNLAAT KSFKVKRSKN ILYMASETIK ELTPSLLDVK NLPVGDGKPK AIDPEMFKLS SVDPSHAAVV NRFWLFGGNE RSLRFIERCI QSFPNFCLLG TEGTPVSWSL MDQTGEMRMA GTLPEYRAQG LVTHAIYQQA QCLLKRGFPV YSHVDPKNQI MQKMSQSLNH VPMPSDWNQW NCEPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate a multitude of substrates to form a variety of N-acylglycines, thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.
Gene References into Functions
  1. Data suggest a catalytic mechanism for GLYAT in which Glu226 functions to deprotonate glycine, facilitating nucleophilic attack on the benzoyl-CoA; studies involve recombinant protein, molecular modeling, and site-directed mutagenesis. PMID: 22071172
Subcellular Location
Mitochondrion.
Protein Families
Glycine N-acyltransferase family
Tissue Specificity
Detected in liver (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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