Recombinant Bovine Interferon-induced GTP-binding protein Mx1 (MX1), partial

Code CSB-YP015249BO
MSDS
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Source Yeast
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Code CSB-EP015249BO
MSDS
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Source E.coli
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Code CSB-EP015249BO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015249BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP015249BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MX1
Uniprot No.
Alternative Names
MX1; Interferon-induced GTP-binding protein Mx1; Myxoma resistance protein 1; Myxovirus resistance protein 1
Species
Bos taurus (Bovine)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Interferon-induced dynamin-like GTPase with antiviral activity against rabies virus (RABV), vesicular stomatitis virus (VSV) and murine pneumonia virus (MPV). Isoform 1 but not isoform 2 shows antiviral activity against vesicular stomatitis virus (VSV).
Gene References into Functions
  1. MX1-a, MX1B and MX2 are affected by the type I IFN pathway during pregnancy and are involved in an immune response to protect the mother and fetus PMID: 26498202
  2. The replication levels of foot-and-mouth disease virus RNA were promoted by silencing bovine Mx1. PMID: 26982472
  3. Overexpression of bovine Mx1 protein inhibits the replication of foot-and-mouth disease virus. PMID: 24294956
  4. Data indicate that the expression profiles of ISG15, MX1, MX2, and OAS1 could be a useful diagnostic biomarker of gestation. PMID: 23384108
  5. The proximal GC boxes were found to be essential for IFN response in the bovine Mx1 promoter with the deletion mutants. PMID: 19203250
  6. Mx1 is cytoplasmic. Mx1B is mainly nuclear. An Arg-rich nuclear localization signal was found in 27 AAs specific for Mx1B. N-terminus-deleted Mx1B is only cytoplasmic. The deleted-Mx1B-expressing cells had positive antiviral activity against VSVDeltaG*-G. PMID: 19951175
  7. A new bovine Mx gene (designated Mx1B) was isolated from the endometrial cDNA library of the early pregnant cow. PMID: 14994826

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Subcellular Location
Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region.; [Isoform 2]: Nucleus.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Tissue Specificity
Ubiquitously expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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