Recombinant Bovine Lysine-specific demethylase 8 (JMJD5)

Code CSB-YP629263BO
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Source Yeast
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Code CSB-EP629263BO
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Source E.coli
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Code CSB-EP629263BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP629263BO
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Source Baculovirus
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Code CSB-MP629263BO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KDM8
Uniprot No.
Alternative Names
KDM8; JMJD5Bifunctional peptidase and arginyl-hydroxylase JMJD5; EC 1.14.11.-; EC 3.4.-.-; JmjC domain-containing protein 5; Jumonji C domain-containing protein 5; L-arginine; 3R)-hydroxylase KDM8
Species
Bos taurus (Bovine)
Expression Region
1-406
Target Protein Sequence
MAGPSTLWET LQALLPHTKE ELKLELGEKV EGSVLMLLQE AAELFLGGQR RECLQTCEVL LDYSWEKLNT GPWQHVDKDW RRVYAFGCLL KAVCLCEPPG DAASVAAALK ACDMGLLMGA AILGDILLKV AAVLQKYLLS GKRPAPGPSQ EPPGTKKARN DHVPIPDVTT ERTVPRLHCP SLQYFKKHFL VPGRPVILEG VANHWPCMKK WSLEYIQEVA GCRTVPVEVG SRYTDEEWSQ TLMTVNEFIS KYIREEPKDI GYLAQHQLFD QIPELKQDIS IPDYCCLGDG EEEEITINAW FGPQGTVSPL HQDPQQNFLA QVMGRKYIRL YSPQESEALY PHDTHLLHNT SQVDVENPDL EKFPRFAEAP FLSCVLSPGE VLFIPVKHWH YVRALDLSFS VSFWWS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage. Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established. Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation. Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition. Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation. Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition. Regulates the circadian gene expression in the liver. Represses the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a catalytically-independent manner. Negatively regulates the protein stability and function of CRY1; required for AMPK-FBXL3-induced CRY1 degradation.
Subcellular Location
Nucleus. Chromosome.
Database Links
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