Recombinant Bovine Lysine-specific demethylase 8(JMJD5)

Code CSB-YP629263BO
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Source Yeast
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Code CSB-EP629263BO
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Source E.coli
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Code CSB-EP629263BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP629263BO
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Source Baculovirus
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Code CSB-MP629263BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names KDM8
Uniprot No. Q1JP61
Alternative Names KDM8; JMJD5Bifunctional peptidase and arginyl-hydroxylase JMJD5; EC 1.14.11.-; EC 3.4.-.-; JmjC domain-containing protein 5; Jumonji C domain-containing protein 5; L-arginine; 3R)-hydroxylase KDM8
Species Bos taurus (Bovine)
Expression Region 1-406
Target Protein Sequence MAGPSTLWET LQALLPHTKE ELKLELGEKV EGSVLMLLQE AAELFLGGQR RECLQTCEVL LDYSWEKLNT GPWQHVDKDW RRVYAFGCLL KAVCLCEPPG DAASVAAALK ACDMGLLMGA AILGDILLKV AAVLQKYLLS GKRPAPGPSQ EPPGTKKARN DHVPIPDVTT ERTVPRLHCP SLQYFKKHFL VPGRPVILEG VANHWPCMKK WSLEYIQEVA GCRTVPVEVG SRYTDEEWSQ TLMTVNEFIS KYIREEPKDI GYLAQHQLFD QIPELKQDIS IPDYCCLGDG EEEEITINAW FGPQGTVSPL HQDPQQNFLA QVMGRKYIRL YSPQESEALY PHDTHLLHNT SQVDVENPDL EKFPRFAEAP FLSCVLSPGE VLFIPVKHWH YVRALDLSFS VSFWWS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation. In a complex with coregulator RCCD1, is also able to demethylate trimethylated 'Lys-36' (H3K36me3) of histone H3. Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, involved in proper mitotic spindle organization and chromosome segregation. Plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability (By similarity). Might function as a protein hydroxylase (Potential). Under stress conditions that cause a DNA damage response, acts as a histone protease by cleaving the N-terminal tail of histone H3 at the carboxyl side of monomethyl-lysine (Kme1) residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage.
Subcellular Location Nucleus, Chromosome
Database Links

KEGG: bta:507905

STRING: 9913.ENSBTAP00000026023

UniGene: Bt.45836

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