Recombinant Bovine Unconventionnal myosin-X(MYO10) ,partial

Code CSB-YP015331BO
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Source Yeast
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Code CSB-EP015331BO
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Source E.coli
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Code CSB-EP015331BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015331BO
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Source Baculovirus
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Code CSB-MP015331BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MYO10
Uniprot No. P79114
Alternative Names MYO10; Unconventional myosin-X; Unconventional myosin-10
Species Bos taurus (Bovine)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation (By similarity). Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate, which are then moved relative to actin filaments. Stimulates the formation and elongation of filopodia. Regulates cell shape, cell spreading and cell adhesion. Plays a role in formation of the podosome belt in osteoclasts.
Gene References into Functions
  1. A Combination of Diffusion and Active Translocation Localizes Myosin 10 to the Filopodial Tip. PMID: 27566544
  2. Single alpha-helices domain of MYO10 unfolds progressively as the length is increased and refolds progressively as the length is reduced. PMID: 25122759
  3. At low optical trap loads, we observed staircase-like processive movements of myosin-10 interacting with the actin filament, consisting of up to six approximately 35-nm steps per binding interaction. PMID: 24753602
  4. Myosin X is monomeric and the band 4.1-ezrin-radixin-moesin (FERM) and pleckstrin homology (PH) domains bind to the head intramolecularly, forming an inhibited conformation. PMID: 21666676
  5. the structural features of the motor and the track that allow myosin X to recognize actin filament bundles PMID: 20858426
  6. myosin X is a membrane-associated molecular motor PMID: 15705568
  7. kinetic analysis of function of Myosin X as a high duty ratio motor PMID: 15961399
  8. the predicted coiled coil of myosin 10 forms a novel elongated structure in which the proximal region is a SAH domain and the distal region is a SAH domain (or has an unknown extended structure) that dimerizes only at its end PMID: 16030012
  9. myosin X recognizes the local structural arrangement of filaments in long bundles, providing a mechanism for sorting cargo to distant target sites. PMID: 18599451

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Subcellular Location Cytoplasm, cytosol, Cell projection, lamellipodium, Cell projection, ruffle, Cytoplasm, cytoskeleton, Cell projection, filopodium tip, Cytoplasm, cell cortex, Cell projection, filopodium membrane, Peripheral membrane protein
Protein Families TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity Detected in kidney, testis, liver, kidney, cerebellum and brain cortex (at protein level).
Database Links

KEGG: bta:281935

STRING: 9913.ENSBTAP00000026812

UniGene: Bt.103339

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