Recombinant Bovine herpesvirus 1.1 Tegument protein VP22 (UL49)

Code CSB-YP341117BJS
MSDS
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Source Yeast
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Code CSB-EP341117BJS
MSDS
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Source E.coli
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Code CSB-EP341117BJS-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341117BJS
MSDS
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Source Baculovirus
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Code CSB-MP341117BJS
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UL49
Uniprot No.
Alternative Names
UL49Tegument protein VP22
Species
Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Expression Region
1-258
Target Protein Sequence
MARFHRPSED EDDYEYSDLW VRENSLYDYE SGSDDHVYEE LRAATSGPEP SGRRASVRAC ASAAAVQPAA RGRDRAAAAG TTVAAPAAAP ARRSSSRASS RPPRAAADPP VLRPATRGSS GGAGAVAVGP PRPRAPPGAN AVASGRPLAF SAAPKTPKAP WCGPTHAYNR TIFCEAVALV AAEYARQAAA SVWDSDPPKS NERLDRMLKS AAIRILVCEG SGLLAAANDI LAARAQRPAA RGSTSGGESR LRGERARP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Tegument protein that plays different roles during the time course of infection. Participates in both the accumulation of viral mRNAs and viral protein translation at late time of infection. Modulates the RNase activity of the virion host shutoff protein UL41 probably to ensure necessary levels of key cellular mRNAs and proteins. Plays a role in microtubule reorganization that occurs after viral infection by stabilizing microtubule network.
Gene References into Functions
  1. These data demonstrate that BoHV-1 VP22 interacts with both CK2 and US3, and that CK2 is the major kinase phosphorylating VP22, with US3 playing a minor role. PMID: 20016039
  2. mitochondrial targeting sequence was identified at the C-terminal 49 amino acids, overlapping the nuclear targeting sequence (VP22) PMID: 15705574
  3. The role of the UL49 gene product, VP22, of bovine herpesvirus type 1 (BoHV-1) in virus replication was characterized with respect to a putative functional interaction of VP22 with the viral glycoprotein E (gE) during BoHV-1 cell-to-cell spread. PMID: 18753236
  4. quantity in the nucleus is precisely regulated at different stages of BHV-1 infection PMID: 19922972
Subcellular Location
Virion tegument. Host cytoplasm. Host nucleus. Host Golgi apparatus.
Protein Families
Alphaherpesvirinae VP22 tegument protein family
Database Links

KEGG: vg:1487397

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