Recombinant Burkholderia vietnamiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B

Code CSB-YP390410BPV
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Source Yeast
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Code CSB-EP390410BPV
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Source E.coli
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Code CSB-EP390410BPV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP390410BPV
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Source Baculovirus
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Code CSB-MP390410BPV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gatB
Uniprot No.
Alternative Names
gatB; Bcep1808_3191Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Species
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Expression Region
1-490
Target Protein Sequence
MTQWEVVIGL ETHAQLSTVS KIFSGAPTQF GAEPNTQACP VDLALPGVLP VLNRGAVERA IRFGLAIGST IAPRSIFARK NYFYPDLPKG YQISQYEIPV VQGGQITIQV PANEKAGKPA YEKTVNLTRA HLEEDAGKSL HEDFAGMTGI DLNRAGTPLL EIVTEPEMRS AAEAVAYAKA LHALVVWLGI CDGNMQEGSF RCDANVSVRP VGQEKFGTRA EIKNLNSFRF LEEAINYEVR RQIELIEDGG EVVQETRLYD PDKRETRSMR SKEDAHDYRY FPDPDLMPLV IGQDWIERVQ AGMPELPAAI QQRFVDQYGV SAYDAGVLTS SKAMAAYFEA VVAKAGAANA KIAANWLMGD VSSQLNRDGI EIDAIPVSAA QLALVLQRIA DGTISNKIAK EIFATIWDEK ATDEGAADRI IDAKGLKQIS DTGALEAIID EVLAANAKSV EEFRAGKEKA FNALIGQAMK ATKGKANPQQ VNELLKKKLG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Protein Families
GatB/GatE family, GatB subfamily
Database Links
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