Recombinant Calpain clp-1 (clp-1), partial

Code CSB-YP335920CXY
MSDS
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Source Yeast
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Code CSB-EP335920CXY
MSDS
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Source E.coli
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Code CSB-EP335920CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335920CXY
MSDS
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Source Baculovirus
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Code CSB-MP335920CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
clp-1
Uniprot No.
Alternative Names
clp-1; C06G4.2Calpain clp-1; EC 3.4.22.-
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates. Required for assembly and maintenance of integrin attachment complexes which are essential for maintenance of adult muscle. Proteolytic activity is activated in response to increased intracellular Ca(2+) levels during cell degeneration and promotes necrotic cell death.
Gene References into Functions
  1. this study reports crystal structures of Clp1 from Caenorhabditis elegans (ceClp1) in a number of nucleotide- and RNA-bound states along the reaction pathway. PMID: 24813946
  2. our analyses highlight the roles of calcium dysregulation and CLP-1 in muscle myopathies and suggest that the atypical calpains could retain conserved PMID: 22479198
Subcellular Location
Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere.
Protein Families
Peptidase C2 family
Tissue Specificity
Expressed in muscle and neuronal tissues. Expressed in the ventral and dorsal nerve cord, intestinal and hypodermal tissues.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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