Recombinant Campylobacter fetus subsp. fetus Bifunctional enzyme IspD/IspF(ispDF)

Product Type Recombinant Protein
Uniprot No. A0RN28
Species Campylobacter fetus subsp. fetus (strain 82-40)
Gene Names ispDF
Protein Names Recommended name: Bifunctional enzyme IspD/IspF Including the following 2 domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase EC= 2.7.7.60 Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase MEP
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).
Protein Families IspD/TarI cytidylyltransferase family, IspD subfamily; IspF family
Database Links

KEGG: cff:CFF8240_0409

Product Details for Different Expression Systems

Product Name Recombinant Campylobacter fetus subsp. fetus Bifunctional enzyme IspD/IspF(ispDF),Yeast
Code CSB-YP368199CWC
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MLDLSLIMLG AGNSTRFGLQ SKKQWLRTGD DPLWLYATKN ISSNYLFKDV IVVSNECEYM RKFSSHFKFI KGGETRQDSL RNAISNINSE FVMVSDIARA DIPKELITKL IESAHNADCI VPALKISDSV IYQNEYINRD ELKLIQTPQL SRTNMLKKAL KTDNIFTDDS SAIKAIGGTV WYIEGDERAK KLTYKDDLKR LNLNSPSNDI FCGNGFDVHA FKEGDFITLC GVKIPYSKAF IAHSDGDVAL HALCDALLGA ASAPDIGELY PDNDSKFKDI DSKILLQNSV NLIRSIGFDI INADITIIAQ SPKISPYKDA MAKIVADILG IPLHKVNIKA TTTEHLGFIG RNEGIAANAI VNLKYFNWRN VL
Expression Region 1-372
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Campylobacter fetus subsp. fetus Bifunctional enzyme IspD/IspF(ispDF),E.coli
Code CSB-EP368199CWC
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MLDLSLIMLG AGNSTRFGLQ SKKQWLRTGD DPLWLYATKN ISSNYLFKDV IVVSNECEYM RKFSSHFKFI KGGETRQDSL RNAISNINSE FVMVSDIARA DIPKELITKL IESAHNADCI VPALKISDSV IYQNEYINRD ELKLIQTPQL SRTNMLKKAL KTDNIFTDDS SAIKAIGGTV WYIEGDERAK KLTYKDDLKR LNLNSPSNDI FCGNGFDVHA FKEGDFITLC GVKIPYSKAF IAHSDGDVAL HALCDALLGA ASAPDIGELY PDNDSKFKDI DSKILLQNSV NLIRSIGFDI INADITIIAQ SPKISPYKDA MAKIVADILG IPLHKVNIKA TTTEHLGFIG RNEGIAANAI VNLKYFNWRN VL
Expression Region 1-372
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Campylobacter fetus subsp. fetus Bifunctional enzyme IspD/IspF(ispDF),Baculovirus
Code CSB-BP368199CWC
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MLDLSLIMLG AGNSTRFGLQ SKKQWLRTGD DPLWLYATKN ISSNYLFKDV IVVSNECEYM RKFSSHFKFI KGGETRQDSL RNAISNINSE FVMVSDIARA DIPKELITKL IESAHNADCI VPALKISDSV IYQNEYINRD ELKLIQTPQL SRTNMLKKAL KTDNIFTDDS SAIKAIGGTV WYIEGDERAK KLTYKDDLKR LNLNSPSNDI FCGNGFDVHA FKEGDFITLC GVKIPYSKAF IAHSDGDVAL HALCDALLGA ASAPDIGELY PDNDSKFKDI DSKILLQNSV NLIRSIGFDI INADITIIAQ SPKISPYKDA MAKIVADILG IPLHKVNIKA TTTEHLGFIG RNEGIAANAI VNLKYFNWRN VL
Expression Region 1-372
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Campylobacter fetus subsp. fetus Bifunctional enzyme IspD/IspF(ispDF),Mammalian cell
Code CSB-MP368199CWC
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MLDLSLIMLG AGNSTRFGLQ SKKQWLRTGD DPLWLYATKN ISSNYLFKDV IVVSNECEYM RKFSSHFKFI KGGETRQDSL RNAISNINSE FVMVSDIARA DIPKELITKL IESAHNADCI VPALKISDSV IYQNEYINRD ELKLIQTPQL SRTNMLKKAL KTDNIFTDDS SAIKAIGGTV WYIEGDERAK KLTYKDDLKR LNLNSPSNDI FCGNGFDVHA FKEGDFITLC GVKIPYSKAF IAHSDGDVAL HALCDALLGA ASAPDIGELY PDNDSKFKDI DSKILLQNSV NLIRSIGFDI INADITIIAQ SPKISPYKDA MAKIVADILG IPLHKVNIKA TTTEHLGFIG RNEGIAANAI VNLKYFNWRN VL
Expression Region 1-372
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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