Recombinant Chicken Estrogen receptor (ESR1), partial

Code CSB-YP007830CH
MSDS
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Source Yeast
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Code CSB-EP007830CH
MSDS
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Source E.coli
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Code CSB-EP007830CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007830CH
MSDS
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Source Baculovirus
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Code CSB-MP007830CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ESR1
Uniprot No.
Alternative Names
ESR1; ESR; NR3A1Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Species
Gallus gallus (Chicken)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Gene References into Functions
  1. The variations of ESR1 or PR expression or dominance of either PR expression is differentially regulated depending on the cell type in the chicken oviduct. PMID: 26519127
  2. differential expression of ER-alpha in both chicken ovaries during development in a cell type-specific distribution, suggesting that these differences may be regarded as an important cause in the process of asymmetric ovarian development in the chicken PMID: 24704443
  3. There were no significant differences in ERalpha mRNA expression during stress induced by fasting and refeeding. PMID: 23342917
  4. cell-specific distribution of ERalpha and AR in the anterior pituitary from adult cockerels by immunohistochemistry PMID: 22453555
  5. Data show that estrogen receptor 1 mRNA is over-expressed in early-stage tumors, suggesting that expression of the oviduct-related genes may be regulated by estrogen. PMID: 21761365
  6. ERalpha plays an important role in sex differentiation of the reproductive tract in female chicken embryos and can mediate xenoestrogen-induced disturbances of sex differentiation. PMID: 21420409
  7. immunohistochemical (IHC) localization of oestrogen receptor alpha was studied in the developing left ovary of 14.5-day-old chick embryos; intracellular localization was cytoplasmic, nuclear, or both PMID: 20887390
  8. Down-regulation of DMRT1 and up-regulation of ER alpha in rooster testes treated with exogenous pEGFP-N1-chicken male hypemethylated (cMHM) plasmid might indicate a novel cMHM non-coding RNA-mediated mechanism in avian sex differentiation. PMID: 19597752
  9. Content of estrogen receptor-alpha (ER-alpha) isoforms related to degree of brain development in chicks (Estrogen receptor alpha) PMID: 14511763
  10. ERalpha is localized in the nucleus of all cell subpopulations of the left ovary and only in epithelial cells of the lacunar channels of the right ovary. PMID: 15704009
  11. The ontogeny of ESR1 and its co-localization with pituitary hormones in the chick pituitary was studied. PMID: 15789219

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR3 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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