Recombinant Chicken Indian hedgehog protein (IHH)

Code CSB-YP839652CH
MSDS
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Source Yeast
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Code CSB-EP839652CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839652CH
MSDS
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Source Baculovirus
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Code CSB-MP839652CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IHH
Uniprot No.
Alternative Names
IHH; Indian hedgehog protein; IHH) [Cleaved into: Indian hedgehog protein N-product; Indian hedgehog protein C-product]
Species
Gallus gallus (Chicken)
Expression Region
24-408
Target Protein Sequence
CGPGRVV GSRRRPPRKL IPLAYKQFSP NVPEKTLGAS GRYEGKIARN SERFKELTPN YNPDIIFKDE ENTGADRLMT QRCKDRLNSL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT TSDRDRNKYG MLARLAVEAG FDWVYYESKA HIHCSVKSEH SAAAKTGGCF PGRALATLEN GARTPLWALR PGQRVLAMDG AGRPTYSDFL AFLDKEPRAL TAFHVIETRQ PPRRLALTPT HLLFVADNAS APAAQFRPTF ASHVQPGHFV LVAVGSGGLQ PAEVVGVRGR TDVGAYAPLT RHGTLVVDDV VASCFALVRE QQLAQMAFWP LRLYHSLLGG PGVQGDGVHW YSGLLYRLGR MLLPPDSFHP LGAPRAES
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
Gene References into Functions
  1. Based on these results, the Ihh signal is essential for balancing chicken chondrocyte proliferation and hypertrophy, and the regulatory function of PTHrP acts in an Ihh-dependent manner. PMID: 23928032
  2. Reduction of Ihh in chicken embryo hindlimbs reduced skeletal muscle mass similar to that seen in Ihh(-/-) mouse embryos PMID: 21683695
  3. BMPs are integrated into the Ihh/PTHrP signaling loop, and a fine balance of BMP signaling is essential for normal chondrocyte maturation and skeletal development. PMID: 12968668
  4. Indian hedgehog and syndecan-3 have roles in chondrocyte proliferation and limb skeletogenesis PMID: 14991716
  5. IHH and FGF8 maybe play different roles and act synergistically to promote chondrogenesis during digit primordia elongation. PMID: 17889828

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Subcellular Location
[Indian hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Indian hedgehog protein C-product]: Secreted, extracellular space.
Protein Families
Hedgehog family
Tissue Specificity
Expressed in developing midgut, lung and cartilage of developing long bones in the limb.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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