Recombinant Chicken Protein Wnt-3a (WNT3A)

Code CSB-YP641688CH
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Source Yeast
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Code CSB-EP641688CH
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Source E.coli
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Code CSB-EP641688CH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP641688CH
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Source Baculovirus
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Code CSB-MP641688CH
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WNT3A
Uniprot No.
Alternative Names
WNT3AProtein Wnt-3a
Species
Gallus gallus (Chicken)
Expression Region
19-352
Target Protein Sequence
SY PIWWSLAIGH QYSSLGTQPI LCGSIPGLVP KQLRFCRNYV EIMPSVAEGV KIGIQECQHQ FRGRRWNCTT VNDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSATICGCDT RHKGSPGEGW KWGGCSEDVE FGSMVSREFA DARENRPDAR SAMNRHNNEA GRTSIIELMH LKCKCHGLSG SCEVKTCWWS QPDFRVIGDY LKDKYDSASE MVVEKHRESR GWVETLRPKY NFFKAPTEKD LVYYENSPNF CEPNPETGSF GTRDRICNVT SHGIDGCDLL CCGRGHNTRT EKRKEKCHCI FHWCCYVRCQ ECIRVYDVHT CK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Regulates chick apical ectodermal ridge formation. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube.
Gene References into Functions
  1. also enhances FGF8 activity while inhibiting BMP5 and WNT3a signaling PMID: 22898776
  2. These results identify Wif-1 as a novel extracellular Wnt modulator in cartilage biology. PMID: 19755491
  3. WNT3A up-regulates WNT/CTNNB1 signaling and regulates FGF signaling during embryonic limb development PMID: 11290326
  4. These results provide the framework for understanding how Sfrps function to regulate Wnt-3a activity in developing embryos and in cancer. PMID: 16425220
  5. there are temporal-specific responses and differential sensitivities to lithium/Wnt-3A exposure during early heart development PMID: 16804895
  6. Alternatively spliced Wnt3a variant 2 transcript is an active form of chick Wnt3a that regulates formation of the apical ectodermal ridge. PMID: 17488271
  7. Wnt-3a is involved in the myogenic induction observed after cholesterol depletion. PMID: 18037380
  8. Cardiac progenitors migrate in highly directed trajectories, which can be controlled by Wnt3a. Ectopic Wnt3a altered movement trajectories and caused cardia bifida. This was rescued by electroporation of DN-Wnt3a into prospective cardiac cells. PMID: 18256196
  9. Studied Wnt3a expression patterns in chick embryo nervous system PMID: 7577679

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Subcellular Location
[Isoform 1]: Secreted, extracellular space, extracellular matrix. Secreted.; [Isoform 2]: Cytoplasm.
Protein Families
Wnt family
Tissue Specificity
Expressed in cornea. Isoform 1 is expressed in the primitive streak, dorsal neural tube, proximal otic vesicle, the apical ectodermal ridge and the epithelium of feather buds.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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