Recombinant Chlamydia trachomatis serovar L2b tRNA (Ile)-lysidine synthase

Code CSB-YP534427DSH
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Source Yeast
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Code CSB-EP534427DSH
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Source E.coli
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Code CSB-EP534427DSH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP534427DSH
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Source Baculovirus
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Code CSB-MP534427DSH
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tilS
Uniprot No.
Alternative Names
tilS; CTLon_0212; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
Species
Chlamydia trachomatis serovar L2b (strain UCH-1/proctitis)
Expression Region
1-321
Target Protein Sequence
MITRLFENDK QLEGFFSSLD KKKKYLLALS GGSDSLFLMY LLKSRAIFFT AVHVDYGWRE TSYQEASDLA ALCEQEQIPF ILDRPEATDP MDSRDIENAA RRYRYELFYR LCKEKCFSGV FLGHHADDQA ETILKRVFEG AHLGNLKGMS AQVMYRDVAL LRPLLHIPKH KIVEALDSHQ VQYVQDITNC NERFLRARMR ERLFPYLQDV FGKNIRDPLL SLAGDSAELR EYLDQQTAPF LLRVVDNERG KLLPIEQELL KTPFLAKWVC KQFFLNEGLV ASKSFLQTVY DHLMTGSTAR LRLRNRTVLV KARGVIIESI Y
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
Subcellular Location
Cytoplasm.
Protein Families
TRNA(Ile)-lysidine synthase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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