Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

Product Type Recombinant Protein
Uniprot No. B1IM69
Species Clostridium botulinum (strain Okra / Type B1)
Gene Names miaB
Protein Names Recommended name: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB EC= 2.-.-.- Alternative name(s): tRNA-i(6)A37 methylthiotransferase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Subcellular Location Cytoplasm
Protein Families Methylthiotransferase family, MiaB subfamily
Database Links

KEGG: cbb:CLD_2839

Product Details for Different Expression Systems

Product Name Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Yeast
Code CSB-YP534250DUJ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNEILNTKDI NAIGEFFIET WGCQMNEEDS EKLSGMLKKE GYIRTEEREN ADVIIFNTCC VRENAELKVY GNLGILKGLK SKNPNLIIAV TGCMMQQKGM AETIKKKFPF VDIIIGTHNL HNFPNYLNEV KKKDTSVLKI QEKEDSIIEN MPIDRKNSMK AFVTIMYGCN NFCTYCIVPY VRGRERSRTP ENIEDEIKKL ISEGYKEITL LGQNVNSYGK DLEPKVTFAE LLERVNTIDG LERVRFMTSH PKDLTDDVIE AIAKCDKLCE QIHLPVQSGS SEILKKMNRH YDREKYLDVV SKIKKLIPNV ALSTDIIVGF PGETEKDFEE TLSLVKEVEY DSAFTFLYSI RKGTPAAKFE DQVPEDVKHK RFNRLVEVVN EISAKKNKAY EGKIEEVLVE GTSKNDENKL MGRTRTGKLV NFIGDKDSIG KLVNVKIIKA NSFSLTGEEI
Expression Region 1-450
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,E.coli
Code CSB-EP534250DUJ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNEILNTKDI NAIGEFFIET WGCQMNEEDS EKLSGMLKKE GYIRTEEREN ADVIIFNTCC VRENAELKVY GNLGILKGLK SKNPNLIIAV TGCMMQQKGM AETIKKKFPF VDIIIGTHNL HNFPNYLNEV KKKDTSVLKI QEKEDSIIEN MPIDRKNSMK AFVTIMYGCN NFCTYCIVPY VRGRERSRTP ENIEDEIKKL ISEGYKEITL LGQNVNSYGK DLEPKVTFAE LLERVNTIDG LERVRFMTSH PKDLTDDVIE AIAKCDKLCE QIHLPVQSGS SEILKKMNRH YDREKYLDVV SKIKKLIPNV ALSTDIIVGF PGETEKDFEE TLSLVKEVEY DSAFTFLYSI RKGTPAAKFE DQVPEDVKHK RFNRLVEVVN EISAKKNKAY EGKIEEVLVE GTSKNDENKL MGRTRTGKLV NFIGDKDSIG KLVNVKIIKA NSFSLTGEEI
Expression Region 1-450
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Baculovirus
Code CSB-BP534250DUJ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNEILNTKDI NAIGEFFIET WGCQMNEEDS EKLSGMLKKE GYIRTEEREN ADVIIFNTCC VRENAELKVY GNLGILKGLK SKNPNLIIAV TGCMMQQKGM AETIKKKFPF VDIIIGTHNL HNFPNYLNEV KKKDTSVLKI QEKEDSIIEN MPIDRKNSMK AFVTIMYGCN NFCTYCIVPY VRGRERSRTP ENIEDEIKKL ISEGYKEITL LGQNVNSYGK DLEPKVTFAE LLERVNTIDG LERVRFMTSH PKDLTDDVIE AIAKCDKLCE QIHLPVQSGS SEILKKMNRH YDREKYLDVV SKIKKLIPNV ALSTDIIVGF PGETEKDFEE TLSLVKEVEY DSAFTFLYSI RKGTPAAKFE DQVPEDVKHK RFNRLVEVVN EISAKKNKAY EGKIEEVLVE GTSKNDENKL MGRTRTGKLV NFIGDKDSIG KLVNVKIIKA NSFSLTGEEI
Expression Region 1-450
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Mammalian cell
Code CSB-MP534250DUJ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNEILNTKDI NAIGEFFIET WGCQMNEEDS EKLSGMLKKE GYIRTEEREN ADVIIFNTCC VRENAELKVY GNLGILKGLK SKNPNLIIAV TGCMMQQKGM AETIKKKFPF VDIIIGTHNL HNFPNYLNEV KKKDTSVLKI QEKEDSIIEN MPIDRKNSMK AFVTIMYGCN NFCTYCIVPY VRGRERSRTP ENIEDEIKKL ISEGYKEITL LGQNVNSYGK DLEPKVTFAE LLERVNTIDG LERVRFMTSH PKDLTDDVIE AIAKCDKLCE QIHLPVQSGS SEILKKMNRH YDREKYLDVV SKIKKLIPNV ALSTDIIVGF PGETEKDFEE TLSLVKEVEY DSAFTFLYSI RKGTPAAKFE DQVPEDVKHK RFNRLVEVVN EISAKKNKAY EGKIEEVLVE GTSKNDENKL MGRTRTGKLV NFIGDKDSIG KLVNVKIIKA NSFSLTGEEI
Expression Region 1-450
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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