Recombinant Corynebacterium glutamicum GDP-mannose-dependent alpha- (1-6)-phosphatidylinositol monomannoside mannosyltransferase (pimB)

Code CSB-YP847774DWY
MSDS
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Source Yeast
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Code CSB-EP847774DWY
MSDS
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Source E.coli
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Code CSB-EP847774DWY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847774DWY
MSDS
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Source Baculovirus
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Code CSB-MP847774DWY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pimB
Uniprot No.
Alternative Names
pimB; Cgl2186; cg2400; GDP-mannose-dependent monoacylated alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase; EC 2.4.1.346; Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-ManT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase
Species
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Expression Region
1-381
Target Protein Sequence
MSASRKTLVV TNDFPPRIGG IQSYLRDFIA TQDPESIVVF ASTQNAEEAH AYDKTLDYEV IRWPRSVMLP TPTTAHAMAE IIREREIDNV WFGAAAPLAL MAGTAKQAGA SKVIASTHGH EVGWSMLPGS RQSLRKIGTE VDVLTYISQY TLRRFKSAFG SHPTFEHLPS GVDVKRFTPA TPEDKSATRK KLGFTDTTPV IACNSRLVPR KGQDSLIKAM PQVIAARPDA QLLIVGSGRY ESTLRRLATD VSQNVKFLGR LEYQDMINTL AAADIFAMPA RTRGGGLDVE GLGIVYLEAQ ACGVPVIAGT SGGAPETVTP ATGLVVEGSD VDKLSELLIE LLDDPIRRAA MGAAGRAHVE AEWSWEIMGE RLTNILQSEP R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2). PimB also catalyzes the addition of a mannosyl residue from GDP-Man to the position 6 of phosphatidyl-myo-inositol bearing an acylated alpha-1,2-linked mannose residue (Ac1PIM1) to generate monoacylated phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2). The addition of the second mannosyl residue by PimB preferentially occurs before the acylation of the mannosyl residue transferred by PimA. Also able to transfer a mannosyl residue from GDP-Man to the position 6 of a phosphatidyl-myo-inositol (PI), but this reaction is very slow.
Gene References into Functions
  1. PimB' (NCgl2106) attaches mannosyl selectively to the 6-OH of the inositol moiety of phosphatidyl-myo-inositol. PMID: 20843801
Protein Families
Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
Database Links

KEGG: cgb:cg2400

STRING: 196627.cg2400

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