Recombinant Cricetulus griseus Interferon-induced protein with tetratricopeptide repeats 2 (IFIT2)

Code CSB-YP011021DXU
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Source Yeast
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Code CSB-EP011021DXU
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Source E.coli
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Code CSB-EP011021DXU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011021DXU
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Source Baculovirus
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Code CSB-MP011021DXU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IFIT2
Uniprot No.
Alternative Names
IFIT2; IFI54; Interferon-induced protein with tetratricopeptide repeats 2; IFIT-2; CL-54 K; Interferon-induced 54 kDa protein; IFI-54K
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Expression Region
2-468
Target Protein Sequence
STTTKKSLE SKLQQLKCHF TWNLMAGDES LDEFEDKVFN KDEFQKRECK ATMCNILAFV KHRRGQNASA LKELEKAEQF IQQQHPDHVE IRNIVTWGNY AWVYYHMGQL EKAQAYLDKV RQVCEKFSSP YRIESPELDC EEGWARLKCT RNQNERVKVC FEKALEKDPK NPEFTSGWAI SNYRLDFWPA QQNAVDSLKQ AIRMSPNSPY VKVLLALKLE MNQENQGKEL VEEALREAPG ETDVLRSAAR FYYKTHDKDR AIQLLSQALE LLPNNAYVYY YIGCFYRSKV LQIDSRRETS QNENREQLLK QAIYYLKKAE ETKEMIKDSC SYLAHLYVLA EQYKEADYYF QKGFKKELTP GLKQLLHLRY GNFQFFQMKC EDKAIHQYLE GVKIRQKTKP KEKMTNKLRF IAERRRSQNG FDSKALHILA FLQELNKESQ QAAKVSERGQ DSERPVFSPS LHEGGNEQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
Subcellular Location
Cytoplasm. Endoplasmic reticulum.
Protein Families
IFIT family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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