Recombinant Danio rerio Glial cell line-derived neurotrophic factor (gdnf)

Code CSB-YP859078DIL
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Source Yeast
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Code CSB-EP859078DIL
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Source E.coli
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Code CSB-EP859078DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859078DIL
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Source Baculovirus
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Code CSB-MP859078DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gdnf
Uniprot No.
Alternative Names
gdnfGlial cell line-derived neurotrophic factor; zGDNF
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
90-235
Target Protein Sequence
S SDVEPQMKRD RVRQKAAANT EKSGGRGRGE RKRSRGRARS RDDRVKGQGR GCLLKEIHLN VTDLDLGYRT KEELIFRYCS GPCHDAETNY DKILNNLTHN KKLDKDTPSR TCCRPIAFDD DISFLDDSLE YHTLKKHSAK KCACV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Essential for the development of the enteric nervous system, but appears dispensable for the development of the kidney and primary motor neuron.
Subcellular Location
Secreted.
Protein Families
TGF-beta family, GDNF subfamily
Tissue Specificity
First expressed at 14 hours post-fertilization (hpf) in the ventral half of anterior somites and in intermediate mesoderm. Ventral somitic expression persists and extends more posteriorly over the next 12 hours. Expressed throughout the ventral trunk meso
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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