Recombinant Danio rerio Mothers against decapentaplegic homolog 1 (smad1)

Code CSB-YP862301DIL
MSDS
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Source Yeast
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Code CSB-EP862301DIL
MSDS
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Source E.coli
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Code CSB-EP862301DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862301DIL
MSDS
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Source Baculovirus
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Code CSB-MP862301DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
smad1
Uniprot No.
Alternative Names
smad1; madh1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; SMAD family member 1; SMAD 1; Smad1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-472
Target Protein Sequence
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE LERALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH HELKALECCE FPFGSKQKDV CINPYHYKRV DSPVLPPVLV PRNSEFNAKL SMLPRFRNPL HQTEPPMPQN ATFPDSFPQQ PANALPFTPN SPTNSYPSSP NSGTGSTATF PHSPSSSDPG SPFQMPETPP PAYMPPEEPM TQDCPQPMDT NLLAPNLPLE ISNRTDVHPV AYQEPKHWCS IVYYELNNRV GEAFLASSTS VLVDGFTDPS NNRNRFCLGL LSNVNRNSTI ENTRRHIGKG VHLYYVGGEV YAECLSDSSI FVQSRNCNYH HGFHPTTVCK IPSRCSLKIF NNQEFAELLA QSVNHGFEAV YELTKMCTIR MSFVKGWGAK YHRQDVTSTP CWIEIHLHGP LQWLDKVLTQ MGSPHNPISS VS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in ventralization. May mediate Bmp2b signaling during embryonic dorsal-ventral pattern formation, and may itself be a transcriptional target for Smad5-mediated Bmp2b signaling.
Gene References into Functions
  1. Data show that interplay of Smad1/5 and MAP kinase signaling system (ERK signalling) is essential for haemogenic endothelium-based haematopoietic stem cell emergence. PMID: 24614941
  2. this study uncovers that smad1 and smad9 act redundantly to each other downstream of smad5 to mediate ventral specification and to regulate embryonic myelopoiesis. PMID: 24488494
  3. that specificity of BMP signaling output, with respect to hematopoiesis, can be explained by differential functions of Smad1 and Smad5. PMID: 17761518
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Dwarfin/SMAD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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