Recombinant Danio rerio Protein Wnt-8a (wnt8a)

Code CSB-YP026143DIL
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Source Yeast
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Code CSB-EP026143DIL
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Source E.coli
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Code CSB-EP026143DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026143DIL
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Source Baculovirus
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Code CSB-MP026143DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
wnt8a
Uniprot No.
Alternative Names
wnt8a; wnt-8; wnt8; Protein Wnt-8a
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
26-359
Target Protein Sequence
VNNFL MTGPKAYLAY TSSVQAGAQS GIEECKHQFA WDRWNCPESA LQLSTHKGLR SATRETAFVH AISAAGVMYT LTKNCSMGDF ENCGCDDSKI GKMGGRGWVW GGCSDNVNFG DRIAKLFVDA LENGHDSRAA VNLHNNEAGR LAVKATLKRT CKCHGLSGSC SIQTCWMQLA DFRDIGSYLK IKHDQARKLE MDKIRMRAGN SADNRGAIAD TFSAVARTEL IFMEDSPDYC VKNLSMGLHG TEGRECLQSG KNLSQWERRS CRRLCHECGL KVEERRIETV SSCNCKFHWC CTVKCETCTQ TVTRYFCAKR HRNRRPHNHS RKRQHTRRG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Required for mesoderm and neural ectoderm patterning during gastrulation. Involved in axis formation during embryonic development, via activation of canonical Wnt/CTNNB1 signaling. May be involved in the specification of the spatial patterns of expression of Gsc and other regulatory genes leading to the establishment of the embryonic axis.
Gene References into Functions
  1. In the Wnt-receiving cells, Wnt8a on cytonemes triggers Wnt/beta-catenin-dependent gene transcription and proliferation. We show that cytoneme-based Wnt transport operates in diverse processes, including zebrafish development, murine intestinal crypt and human cancer organoids, demonstrating that Wnt transport by cytonemes and its control via the Ror2 pathway is highly conserved in vertebrates PMID: 30060804
  2. The results demonstrate that the argon plasma jet has no adverse effects on fin regeneration and embryogenesis in zebrafish, and does not interrupt the (Wnt)/beta-Catenin-signaling pathways. PMID: 28706297
  3. These results indicated that maternal wnt8a is dispensable for the initial dorsal determination, but cooperates with zygotic wnt8a for ventrolateral and posterior tissue formation. PMID: 29208373
  4. Wnt signaling balances specification of the cardiac and pharyngeal muscle fields. PMID: 28087459
  5. Data suggest that Wnt3, Wnt3a, and Wnt8a bind to their respective receptors (Fz8, Lrp6, and Lypd6) in ordered plasma membrane environments; ordered plasma membrane environments appear to be essential for binding of Wnt proteins to their receptor complexes and stimulation of downstream signaling activity. PMID: 28626941
  6. Wnt8, in addition to its well-established role in posterior mesoderm patterning, also plays a later role as a factor that expands the renal progenitor pool prior to kidney morphogenesis. PMID: 28359809
  7. Cortical depth and differential transport of vegetally localized dorsal and germ line determinants, Wnt8a, grip2a, and Dazl, in the zebrafish embryo have been presented. PMID: 26528729
  8. Gain of function of Wnt8a results in the accumulation of beta-catenin into the nuclei of all cells of the gastrula from the margin to the animal pole. PMID: 25371059
  9. A filopodia-based transport system for Wnt8a controls anteroposterior patterning of the neural plate during vertebrate gastrulation. PMID: 25556612
  10. Wnt limits its own signaling activity in part via up-regulation of a transporter, slc35c1 that promotes terminal fucosylation and thereby limits Wnt activity. PMID: 25238963
  11. Dynamic association with donor cell filopodia and lipid-modification are essential features of Wnt8a during patterning of the zebrafish neuroectoderm. PMID: 24427298
  12. post-transcriptional control of wnt8a is essential to fine tune the balance of the signaling outputs of the complex wnt8a locus, which is essential to zebrafish axis development PMID: 24333179
  13. A novel role for Eaf1 and Eaf2 in inhibiting canonical Wnt/beta-catenin signaling, which might form the mechanistic basis for Eaf1 and Eaf2 tumor suppressor activity. PMID: 23364330
  14. Wnt8a expression pattern reflects complex interactions of multiple regulatory inputs. PMID: 22473868
  15. 2-OST functions within the Wnt pathway, downstream of Wnt ligand signaling and upstream of Gsk3beta and beta-catenin intracellular localization and function PMID: 22357927
  16. It was shown that Wnt3a-Wnt8a/beta-catenin signaling directly regulates ciliogenic transcription factor foxj1a expression and ciliogenesis in zebrafish Kupffer's vesicle. PMID: 22190638
  17. wnt8a expression marks Nodal-independent tail mesoderm formation and that Ntl/Bra predominantly regulates wnt8a in paraxial mesoderm progenitors. PMID: 21384472
  18. Kzp controls canonical Wnt8 signaling to modulate dorsoventral patterning during zebrafish gastrulation PMID: 20978132
  19. Wnt8 and BMP signaling have independent roles during vertebrate ventrolateral mesoderm development that can be identified through loss-of-function analysis. PMID: 20845427
  20. wnt8 has a role as neural posteriorizing factor and Frizzled 8c and Frizzled 9 are its functional receptors PMID: 12676325
  21. Loss-of-function experiments reveal that bone morphogenetic protein (BMP), Nodal and Wnt8 signalling pathways are required for tail development PMID: 12879074
  22. Wnt8 achieves restriction on size of dorsal organizer by regulating the early expression of Vent and Vox. PMID: 15269175
  23. FoxA3 and goosecoid cooperate to promote formation of anterior neural tissue by protecting, as early as blastula stage, presumptive anterior neural cells from an irreversible caudalization by the posteriorizing factor Wnt8a. PMID: 16364286
  24. In addition to its role in midbrain-hindbrain formation, we have shown that gbx1 is a novel mediator of Wnt8 signaling during hindbrain patterning. PMID: 19341460

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
Expressed in the margin of the pregastrula embryo destined to be the future mesoderm.
Database Links

KEGG: dre:30122

STRING: 7955.ENSDARP00000116057

UniGene: Dr.309

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