Recombinant Delftia acidovorans Ketol-acid reductoisomerase(ilvC)

Product Type Recombinant Protein
Uniprot No. A9BME9
Species Delftia acidovorans (strain DSM 14801 / SPH-1)
Gene Names ilvC
Protein Names Recommended name: Ketol-acid reductoisomerase EC= 1.1.1.86 Alternative name(s): Acetohydroxy-acid isomeroreductase Alpha-keto-beta-hydroxylacil reductoisomerase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: dac:Daci_4865

STRING: 398578.Daci_4865

Product Details for Different Expression Systems

Product Name Recombinant Delftia acidovorans Ketol-acid reductoisomerase(ilvC),Yeast
Code CSB-YP433532DHV
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MKVFYDKDCD LSLIKGKTVA IIGYGSQGHA HAQNLNESGV KVVVGLRKGG ASWDKVAKAG LTVAEVDDAV KSADLVMILL PDENIPEVYN NNVAPNIKQG ATLAFAHGFN IHYNQVVPRA DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVYQDKSGKA RDLALSYAMA NGGGKGGIIE TNFKEETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE GGIANMNYSI SNNAEYGEYV TGTEVINDKS REAMRAALKR IQTGEYAKMF IQEGRTNYPS MTARRRMNAD HAIEKVGGQL RAMMPWISKN KLVDQSRN
Expression Region 1-338
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Delftia acidovorans Ketol-acid reductoisomerase(ilvC),E.coli
Code CSB-EP433532DHV
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MKVFYDKDCD LSLIKGKTVA IIGYGSQGHA HAQNLNESGV KVVVGLRKGG ASWDKVAKAG LTVAEVDDAV KSADLVMILL PDENIPEVYN NNVAPNIKQG ATLAFAHGFN IHYNQVVPRA DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVYQDKSGKA RDLALSYAMA NGGGKGGIIE TNFKEETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE GGIANMNYSI SNNAEYGEYV TGTEVINDKS REAMRAALKR IQTGEYAKMF IQEGRTNYPS MTARRRMNAD HAIEKVGGQL RAMMPWISKN KLVDQSRN
Expression Region 1-338
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Delftia acidovorans Ketol-acid reductoisomerase(ilvC),Baculovirus
Code CSB-BP433532DHV
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MKVFYDKDCD LSLIKGKTVA IIGYGSQGHA HAQNLNESGV KVVVGLRKGG ASWDKVAKAG LTVAEVDDAV KSADLVMILL PDENIPEVYN NNVAPNIKQG ATLAFAHGFN IHYNQVVPRA DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVYQDKSGKA RDLALSYAMA NGGGKGGIIE TNFKEETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE GGIANMNYSI SNNAEYGEYV TGTEVINDKS REAMRAALKR IQTGEYAKMF IQEGRTNYPS MTARRRMNAD HAIEKVGGQL RAMMPWISKN KLVDQSRN
Expression Region 1-338
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Delftia acidovorans Ketol-acid reductoisomerase(ilvC),Mammalian cell
Code CSB-MP433532DHV
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MKVFYDKDCD LSLIKGKTVA IIGYGSQGHA HAQNLNESGV KVVVGLRKGG ASWDKVAKAG LTVAEVDDAV KSADLVMILL PDENIPEVYN NNVAPNIKQG ATLAFAHGFN IHYNQVVPRA DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVYQDKSGKA RDLALSYAMA NGGGKGGIIE TNFKEETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE GGIANMNYSI SNNAEYGEYV TGTEVINDKS REAMRAALKR IQTGEYAKMF IQEGRTNYPS MTARRRMNAD HAIEKVGGQL RAMMPWISKN KLVDQSRN
Expression Region 1-338
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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