Recombinant Dickeya dadantii Pectate lyase E (pelE)

Code CSB-YP314918DKD
MSDS
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Source Yeast
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Code CSB-EP314918DKD
MSDS
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Source E.coli
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Code CSB-EP314918DKD-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP314918DKD
MSDS
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Source Baculovirus
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Code CSB-MP314918DKD
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pelE
Uniprot No.
Alternative Names
pelE; Dda3937_03371; Pectate lyase E; EC 4.2.2.2
Species
Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Expression Region
42-404
Target Protein Sequence
ASLQTTKAT EAASTGWATQ SGGTTGGAKA SSSKIYAVKS ISEFKAALNG TDSSPKIIQV TGAIDISGGK AYTSFDDQKA RSQISIPSNT TIIGIGNKGK FTNGSLVVKG VSNVILRNLY IETPVDVAPH YEEGDGWNAE WDAVVIDSTD HVWVDHVTIS DGSLTDDKYT TKNGEKYVQH DGSLDIKRGS DYVTVSNSRF ELHDKTILIG HSDNNGSQDA GKLRVTFHNN LFDRVGERTP RVRFGSVHAY NNVYVGDVNH KAYRYQYSFG IGTSGSLLSE SNAFTIDNMK KISGRDKECS VVKAFNGKIF SDKGSIINGA SYNLNGCGFG FSAYSAKIPY KYSAQTITTS LANSISSNAG YGKL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in maceration and soft-rotting of plant tissue. Pectate lyases have been implicated as pathogenicity factors which induce maceration or rotting of plant tissue. PelE is sufficient to induce these effects under laboratory conditions.
Subcellular Location
Secreted.
Protein Families
Polysaccharide lyase 1 family, PLBC subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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