Recombinant Dictyostelium discoideum Histone H2A.x(H2AX)

Code CSB-YP681774DKK
Size Pls inquire other sizes
Source Yeast
Code CSB-EP681774DKK
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Source E.coli
Code CSB-EP681774DKK-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP681774DKK
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Source Baculovirus
Code CSB-MP681774DKK
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names H2AX
Uniprot No. Q54WG6
Species Dictyostelium discoideum (Slime mold)
Expression Region 1-154
Target Protein Sequence MSETKPASSK PAAAAKPKKV IPRVSRTGEP KSKPESRSAR AGITFPVSRV DRLLREGRFA PRVESTAPVY LAAVLEYLVF EILELAHNTC SISKKTRITP QHINWAVGND LELNSLFQHV TIAYGGVLPT PQQSTGEKKK KPSKKAAEGS SQIY
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized Form Lyophilized powder
Buffer before Lyophilization Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity). Phosphorylation of H2AX seems to be performed by atr1 and/or possibly dnapkcs, as ATM ortholog is absent in the genome of this organism.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

KEGG: ddi:DDB_G0279667

STRING: 44689.DDB0216271

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