Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32)

Code CSB-YP681770DKK
Size Pls inquire other sizes
Source Yeast
Code CSB-EP681770DKK
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Source E.coli
Code CSB-EP681770DKK-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP681770DKK
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Source Baculovirus
Code CSB-MP681770DKK
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names bud32
Uniprot No. Q54W07
Species Dictyostelium discoideum (Slime mold)
Expression Region 1-252
Target Protein Sequence MDANECSNSE NSATTTIPKS ASPSTTISTK VVKLDEIGIL ISQGAEAKTY ETDLYGLKCI VKERFSKAYR HPILDQKISS KRILQEVRSL NKCKKKGIQV PSLYLVDIGN NRIYMEFIKG ETVKHYLYKN QESTQHQNQI ESIMKELGNQ IGIIHEMNVI HGDLTTSNML LRESTNELVF IDFGLSYTSN SVEDKAVDLY VLERAFISTH PNSEQLFQTI LSNYELTSSN SKIVIQKLNQ VRLRGRKKTC FG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Bud32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit osgep (By similarity).
Subcellular Location Nucleus
Protein Families Protein kinase superfamily, BUD32 family
Database Links

KEGG: ddi:DDB_G0279977

STRING: 44689.DDB0216402

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