Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32)

Product Type Recombinant Protein
Uniprot No. Q54W07
Abbreviation bud32
Species Dictyostelium discoideum (Slime mold)
Gene Names bud32
Protein Names Recommended name: Probable serine/threonine-protein kinase BUD32 homolog EC= 2.7.11.1
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Bud32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit osgep (By similarity).
Subcellular Location Nucleus
Protein Families Protein kinase superfamily, BUD32 family
Database Links

KEGG: ddi:DDB_G0279977

STRING: 44689.DDB0216402

Product Details for Different Expression Systems

Product Name Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32),Yeast
Code CSB-YP681770DKK
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDANECSNSE NSATTTIPKS ASPSTTISTK VVKLDEIGIL ISQGAEAKTY ETDLYGLKCI VKERFSKAYR HPILDQKISS KRILQEVRSL NKCKKKGIQV PSLYLVDIGN NRIYMEFIKG ETVKHYLYKN QESTQHQNQI ESIMKELGNQ IGIIHEMNVI HGDLTTSNML LRESTNELVF IDFGLSYTSN SVEDKAVDLY VLERAFISTH PNSEQLFQTI LSNYELTSSN SKIVIQKLNQ VRLRGRKKTC FG
Expression Region 1-252
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32),E.coli
Code CSB-EP681770DKK
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDANECSNSE NSATTTIPKS ASPSTTISTK VVKLDEIGIL ISQGAEAKTY ETDLYGLKCI VKERFSKAYR HPILDQKISS KRILQEVRSL NKCKKKGIQV PSLYLVDIGN NRIYMEFIKG ETVKHYLYKN QESTQHQNQI ESIMKELGNQ IGIIHEMNVI HGDLTTSNML LRESTNELVF IDFGLSYTSN SVEDKAVDLY VLERAFISTH PNSEQLFQTI LSNYELTSSN SKIVIQKLNQ VRLRGRKKTC FG
Expression Region 1-252
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32),Baculovirus
Code CSB-BP681770DKK
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDANECSNSE NSATTTIPKS ASPSTTISTK VVKLDEIGIL ISQGAEAKTY ETDLYGLKCI VKERFSKAYR HPILDQKISS KRILQEVRSL NKCKKKGIQV PSLYLVDIGN NRIYMEFIKG ETVKHYLYKN QESTQHQNQI ESIMKELGNQ IGIIHEMNVI HGDLTTSNML LRESTNELVF IDFGLSYTSN SVEDKAVDLY VLERAFISTH PNSEQLFQTI LSNYELTSSN SKIVIQKLNQ VRLRGRKKTC FG
Expression Region 1-252
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase BUD32 homolog(bud32),Mammalian cell
Code CSB-MP681770DKK
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDANECSNSE NSATTTIPKS ASPSTTISTK VVKLDEIGIL ISQGAEAKTY ETDLYGLKCI VKERFSKAYR HPILDQKISS KRILQEVRSL NKCKKKGIQV PSLYLVDIGN NRIYMEFIKG ETVKHYLYKN QESTQHQNQI ESIMKELGNQ IGIIHEMNVI HGDLTTSNML LRESTNELVF IDFGLSYTSN SVEDKAVDLY VLERAFISTH PNSEQLFQTI LSNYELTSSN SKIVIQKLNQ VRLRGRKKTC FG
Expression Region 1-252
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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