Recombinant Dictyostelium discoideum Protein IMPACT homolog (impact)

Code CSB-YP684596DKK
MSDS
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Source Yeast
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Code CSB-EP684596DKK
MSDS
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Source E.coli
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Code CSB-EP684596DKK-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP684596DKK
MSDS
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Source Baculovirus
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Code CSB-MP684596DKK
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
impact
Uniprot No.
Alternative Names
impact; DDB_G0287757; Protein IMPACT homolog
Species
Dictyostelium discoideum (Slime mold)
Expression Region
1-345
Target Protein Sequence
MNEEQENEIL AISSIYPDSF SEIETIVGEE ENDGFEDNDS QYSKIYQVII TPNISCLDEN SLEDNNNSNN NNNNNNNNNN NGLIIDTSYD YLIYFKFKYT KEYPSNEPPI ISIKATWLQK SDQSILLSHL EDLWNQNELV IFQMISWLQE ESIEILNNHY KSIKKFSHYH LKKSLNTQQL NQNQNQFENQ NQNQNQNQNQ NQNKEKEKEK VPTIYTGQSV TEKKSKFQAH LAIVHSEREV QLVLNQLLSF KKIYEATHNM YAYRFQLENG EINEYYNDDG EDGAGDKMLF TLSKNQAKEI LIVCTRWFGG ILLGGRRYVH IVNTTKDILN LYNTNSLSQC SLEFN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Translational regulator that ensures constant high levels of translation under amino acid starvation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis.
Subcellular Location
Cytoplasm.
Protein Families
IMPACT family
Database Links
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