Recombinant Drosophila melanogaster Protein Wnt-4 (Wnt4), partial

Code CSB-YP026137DLU
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Source Yeast
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Code CSB-EP026137DLU
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Source E.coli
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Code CSB-EP026137DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026137DLU
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Source Baculovirus
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Code CSB-MP026137DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wnt4
Uniprot No.
Alternative Names
Wnt4; Wnt-4; CG4698; Protein Wnt-4; dWnt-4
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Acts downstream of homeotic complex genes in the visceral mesoderm and is required for embryonic segmentation. Also required for cell movement and FAK regulation during ovarian morphogenesis.
Gene References into Functions
  1. At different points of development, dWnt4 switches from using the non-canonical pathway components to using a beta-catenin-dependent canonical pathway in the escort cells to facilitate the proper differentiation of Germline stem cells. PMID: 29370168
  2. disruption of Wnt4 and components of the canonical Wnt pathway results in a complex germ cell phenotype marked by an expansion of germline stem cell-like cells, pre-cystoblasts and cystoblasts in young females. PMID: 27364467
  3. Wnt4 and the canonical Wnt signaling pathway are essential for ostia formation in Drosophila PMID: 26994311
  4. dSETDB1 is required for the expression of a Wnt ligand, Drosophila Wingless type mouse mammary virus integration site number 4 (dWnt4) in the somatic niche. PMID: 27019121
  5. this study identifies a transcriptional switch involving the kinase Btk29A/Btk and its phosphorylation target, beta-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of piwi expression. PMID: 24436419
  6. Wg and Wnt4 act redundantly in planar cell polarity determination in Drosophila. PMID: 23912125
  7. We re-evaluated the expression pattern of DWnt4 during embryogenesis and show that transcripts are not restricted to the dorsal ectoderm but are also present in the cardiogenic mesoderm. PMID: 22371299
  8. In Drosophila, Otk interacts with Wnt4 and opposes canonical Wnt signalling in embryonic patterning. PMID: 21772251
  9. It works as a negative signal in neural connectivity. (review) PMID: 21089332
  10. DWnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. PMID: 11970894
  11. DWnt4 antagonizes the polarizing effect of four-jointed PMID: 15986451
  12. in DWnt4 mutants, ventral retinal axons misprojected to the dorsal lamina PMID: 16369482

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Wnt family
Database Links

KEGG: dme:Dmel_CG4698

STRING: 7227.FBpp0088345

UniGene: Dm.341

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