Recombinant Drosophila melanogaster Protein argonaute-2 (AGO2), partial

Code CSB-YP892900DLU
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Source Yeast
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Code CSB-EP892900DLU
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Source E.coli
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Code CSB-EP892900DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892900DLU
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Source Baculovirus
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Code CSB-MP892900DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGO2
Uniprot No.
Alternative Names
AGO2; CG7439Protein argonaute-2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.
Gene References into Functions
  1. Results provide evidence for an Ago2-interaction network participating in dosage compensation in drosophila. PMID: 29921620
  2. Molecular evolution of AGO2 glutamine-rich repeat region PMID: 27317784
  3. Mutation of T1149 or R1158 in the conserved PIWI domain causes AGO2 protein instability, but only T1149 affects RNAi activity. Mass spec analysis shows that several proteasome components co-purify with both wildtype and mutant AGO2, and knockdown of two proteasome pathway components results in AGO2 protein accumulation. PMID: 28087276
  4. We speculate that the repeated evolution of testis specificity in obscura group Ago2 genes, combined with their dynamic turnover and strong signatures of adaptive evolution, may be associated with highly derived roles in the suppression of transposable elements or meiotic drive PMID: 27535930
  5. Study shows that the Cricket Paralysis virus suppressor of RNA silencing, CrPV-1A but not B2 strongly interfere with the Ago-2-dependent miRNA silencing in Drosophila. PMID: 25793377
  6. siRNA biogenesis does not stabilize AGO2 in Drosophila. PMID: 23708604
  7. The results of this study found that mutations in Drosophila Argonaute 2 (Ago2) resulted in exacerbated transposon expression in the brain, progressive and age-dependent memory impairment, and shortened lifespan PMID: 23563579
  8. two new nuclear roles for Ago-2: one in pre-mRNA splicing and one in transcriptional repression. PMID: 23392611
  9. Highlighting its role in antiviral defense, fly Ago2 dissociates so slowly from extensively complementary target RNAs that essentially every fully paired target is cleaved. Conversely, mouse AGO2, which mainly mediates miRNA-directed repression, dissociates rapidly and with similar rates for fully paired and seed-matched targets. PMID: 23178124
  10. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. PMID: 22916019
  11. GRR variation does not modulate an essential function of Ago2 and the amino-terminal domain of Ago2 is subject to rapid evolution PMID: 21253006
  12. Data suggest that recent independent selective sweeps in AGO2 have reduced genetic variation across a region of more than 50 kbp in Drosophila melanogaster, D. simulans, and D. yakuba. PMID: 20978039
  13. three-dimensional nuclear magnetic resonance structure of the Drosophila melanogaster Ago2 PAZ domain PMID: 14615801
  14. Crystal structure of the PAZ domain PMID: 14625589
  15. describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides PMID: 15156196
  16. NMR assignment of the Drosophila Argonaute2 PAZ domain PMID: 15213449
  17. AGO2 is an essential component for siRNA-directed RNA interference (RNAi) response and is required for the unwinding of siRNA duplex and in consequence assembly of siRNA into RISC in Drosophila embryos. PMID: 15231716
  18. found homology between the PIWI domain of Ago-2 and endonuclease V and identified potential active-site amino acid residues within the PIWI domain of Ago-2 PMID: 15452342
  19. Depletion of AGO2, the essentiual factor for siRNA-directed RNAi, resulted in EFGP mRNA stabilization with concomitant shortening of the EFGP mRNA poly(A)tail. PMID: 15987811
  20. nuclear division is asynchronous in mutant ago-2 embryos and there are defects in chromosome condensation, nuclear kinesis, and assembly of spindle apparatus PMID: 16024657
  21. Results show both strands of siRNA get loaded onto Ago2 protein in RNA interference. PMID: 16271385
  22. Ago2 is the catalytic component of the RNAi enzyme complex (RISC) and directly receives the double-stranded siRNA from the RISC assembly machinery. PMID: 16271386
  23. A genome-wide analysis of mRNAs regulated by AGO2 was performed. PMID: 16581772
  24. Argonaute 2 is essential for antiviral defense in adult Drosophila melanogaster. PMID: 17079687
  25. all Argonautes elevate mature miRNA expression posttranscriptionally, independent of RNase activity. we identify a role for (Ago2) in cleaving the pre-miRNA to an additional processing intermediate, termed Ago2-cleaved precursor miRNA PMID: 18083100
  26. Sequence requirements for loading small RNAs into functional Argonaute complexes narrow the range of miRNA and miRNA 5' ends that accumulate in flies. PMID: 18207740
  27. findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila PMID: 18463636
  28. processing of cis-NATs by RNA interference (RNAi) seems to be actively restricted, and the selected loci are enriched for nucleic acid-based functions and include Argonaute-2 (AGO2) itself PMID: 18500351
  29. Ago2-RISC represses translation by competitively blocking the interaction of eIF4E with eIF4G and inhibiting the cap function. PMID: 19268617

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Subcellular Location
Cytoplasm, Cytoplasmic ribonucleoprotein granule.
Protein Families
Argonaute family, Ago subfamily
Database Links

KEGG: dme:Dmel_CG7439

STRING: 7227.FBpp0075313

UniGene: Dm.9677

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