Recombinant Drosophila melanogaster Frequenin-1 (Frq1)

Code CSB-YP339793DLU
MSDS
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Source Yeast
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Code CSB-EP339793DLU
MSDS
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Source E.coli
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Code CSB-EP339793DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339793DLU
MSDS
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Source Baculovirus
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Code CSB-MP339793DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Frq1
Uniprot No.
Alternative Names
Frq1; Frq; CG5744Frequenin-1; d-FRQ
Species
Drosophila melanogaster (Fruit fly)
Expression Region
2-187
Target Protein Sequence
GKKSSKLKQ DTIDRLTTDT YFTEKEIRQW HKGFLKDCPN GLLTEQGFIK IYKQFFPQGD PSKFASLVFR VFDENNDGSI EFEEFIRALS VTSKGNLDEK LQWAFRLYDV DNDGYITREE MYNIVDAIYQ MVGQQPQSED ENTPQKRVDK IFDQMDKNHD GKLTLEEFRE GSKADPRIVQ ALSLGGG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ca(2+)-dependent modulation of synaptic efficacy.
Gene References into Functions
  1. [review] Stimulation protocols, sources of calcium and expression patterns (presynaptic vs postsynaptic) of Frq may result in the activation of various protein targets, leading to different synaptic effects. PMID: 22396213
  2. Frq modulates Ca(2+) entry through a functional interaction with the alpha(1) voltage-gated Ca(2+)-channel subunit; this interaction regulates neurotransmission and nerve-terminal growth. PMID: 19861494
  3. Synonymous substitutions have been prevalent in the evolution of the coding region of frq1 and frq2, indicating the presence of strong functional constraints following gene duplication. PMID: 20170488
  4. We conclude that Frqs have two distinct functions: one in neurotransmission, regulating the probability of release per synapse, and another in axonal growth and bouton formation. PMID: 17970740
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
Recoverin family
Tissue Specificity
Enriched in synapses, such as the motor nerve endings at neuromuscular junctions.
Database Links

KEGG: dme:Dmel_CG5744

STRING: 7227.FBpp0074319

UniGene: Dm.35315

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