Recombinant Drosophila melanogaster Homeobox protein abdominal-A (abd-A), partial

Code CSB-YP333458DLU
MSDS
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Source Yeast
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Code CSB-EP333458DLU
MSDS
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Source E.coli
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Code CSB-EP333458DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333458DLU
MSDS
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Source Baculovirus
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Code CSB-MP333458DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
abd-A
Uniprot No.
Alternative Names
abd-A; CG10325Homeobox protein abdominal-A
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for segmental identity of the second through eighth abdominal segments. Once a pattern of abd-A expression is turned on in a given parasegment, it remains on the more posterior parasegment, so that the complex pattern of expression is built up in the successive parasegments. Appears to repress expression of Ubx whenever they appear in the same cell, but abd-A is repressed by Abd-B only in the eight and ninth abdominal segments.
Gene References into Functions
  1. We also observed that these functions of abd-A are required in its exclusive as well as the coexpression domain with that of Abd-B PMID: 25340649
  2. This "iab-8 RNA" produces a micro RNA to repress abd-A, but also has a second, redundant repression mechanism that acts only "in cis." Transcriptional interference with the abd-A promoter is the most likely mechanism PMID: 22654672
  3. Abdominal-A uses both Pax2-dependent and Pax2-independent mechanisms to stimulate rhomboid Cis-regulatory-modules activity to induce proper oenocyte numbers. PMID: 22438572
  4. The results uncover redundancy, interactivity, and multifunctionality of protein domains as salient features underlying overall AbdA protein activity PMID: 22046139
  5. Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen. PMID: 20875816
  6. in order to make an oenocyte, abdA regulates just one principal target, rho, that acts at the top of a complex hierarchy of cell-differentiation genes PMID: 12050142
  7. abd-A expression in the dorsal vessel was detected only in the heart, and overexpression of abd-A induced heart fate in the aorta in a cell-autonomous manner. PMID: 12397110
  8. In the abdomen, grainy head CG5058-PA terminates neural proliferation by regulating the competence of neuroblasts to undergo apoptosis in response to Abdominal-A expression PMID: 16049114
  9. The relationship between Abd-B and abd-A in the female genital disc is opposite to that of the embryonic epidermis, and contravenes the rule that posteriorly expressed Hox genes downregulate more anterior ones. PMID: 16319117

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Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Database Links

KEGG: dme:Dmel_CG10325

STRING: 7227.FBpp0082829

UniGene: Dm.3286

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