Recombinant Drosophila melanogaster Mitogen-activated protein kinase ERK-A (rl)

Code CSB-YP336719DLU
MSDS
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Source Yeast
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Code CSB-EP336719DLU
MSDS
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Source E.coli
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Code CSB-EP336719DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336719DLU
MSDS
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Source Baculovirus
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Code CSB-MP336719DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rl
Uniprot No.
Alternative Names
rl; ERKa; MAPK; CG12559Mitogen-activated protein kinase ERK-A; EC 2.7.11.24; Extracellular-regulated kinase A; Protein rolled
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-376
Target Protein Sequence
MEEFNSSGSV VNGTGSTEVP QSNAEVIRGQ IFEVGPRYIK LAYIGEGAYG MVVSADDTLT NQRVAIKKIS PFEHQTYCQR TLREITILTR FKHENIIDIR DILRVDSIDQ MRDVYIVQCL METDLYKLLK TQRLSNDHIC YFLYQILRGL KYIHSANVLH RDLKPSNLLL NKTCDLKICD FGLARIADPE HDHTGFLTEY VATRWYRAPE IMLNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL NHILGVLGSP SRDDLECIIN EKARNYLESL PFKPNVPWAK LFPNADALAL DLLGKMLTFN PHKRIPVEEA LAHPYLEQYY DPGDEPVAEV PFRINMENDD ISRDALKSLI FEETLKFKER QPDNAP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway to regulate proliferation, differentiation and effect cell fate decisions in various tissues. Required downstream of phl/Raf in the sev/sevenless, tor/torso, and EGF receptor homolog Egfr signal transduction pathways. Required for embryonic epithelial tissue repair. During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis.
Gene References into Functions
  1. These results identify a role for the Exon Junction Complex (EJC) in the splicing of a subset of transcripts and suggest that RAS/MAPK signaling depends on the regulation of MAPK levels by the EJC. PMID: 20946983
  2. These data suggest a model in which ERK map kinase phosphorylation must be spatially coupled to integrin-mediated DIM-7 activation to make a complex that can be imported efficiently. PMID: 17699602
  3. Data show that Fak56 suppresses MAPK activation in neuromuscular junction growth. PMID: 18925939
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Tissue Specificity
In third instar larvae, expressed in eye imaginal disks. In adults, expressed in head and body.
Database Links
CUSABIO guaranteed quality
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