Recombinant Drosophila melanogaster Myosin regulatory light chain sqh (sqh)

Code CSB-YP336720DLU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP336720DLU
MSDS
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Source E.coli
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Code CSB-EP336720DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336720DLU
MSDS
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Source Baculovirus
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Code CSB-MP336720DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sqh
Uniprot No.
Alternative Names
sqh; CG3595; Myosin regulatory light chain sqh; Myosin regulatory light chain; nonmuscle; MRLC-C; Protein spaghetti-squash
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-174
Target Protein Sequence
MSSRKTAGRR ATTKKRAQRA TSNVFAMFDQ AQIAEFKEAF NMIDQNRDGF VEKEDLHDML ASLGKNPTDD YLDGMMNEAP GPINFTMFLT LFGERLQGTD PEDVIKNAFG CFDEENMGVL PEDRLRELLT TMGDRFTDED VDEMYREAPI KNGLFDYLEF TRILKHGAKD KDEQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for cytokinesis, could regulate contractile ring function.
Gene References into Functions
  1. inclusion body myopathy type 3 disease state in heterozygote Drosophila was examined in order to gain a mechanistic understanding of myosin E701K pathogenicity. PMID: 28258125
  2. Computer simulation coupled with laser ablation suggests that Dunk-dependent maintenance of cortical myosin enables mechanical tension build-up, thereby providing a mechanism to guide myosin flow and define the hexagonal symmetry of the furrows. PMID: 27226317
  3. mechanical forces regulate myosin dynamics during embryonic wound repair. PMID: 28202603
  4. the regulation of NMII takes place downstream of guidance receptor signalling and is critical to ensure efficient collective migration. PMID: 27034137
  5. Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis PMID: 26780298
  6. evidence for mechanosensitivity of cell-cell junctions and implications that myosin-mediated tension can prevent Snail-driven Eepithelial-mesenchymal transitions PMID: 26754645
  7. Study indicated that Sqh is crucial to regulate the stability of the transcription factor Cubitus interruptus (Ci) in Hedgehog (Hh) pathway. PMID: 26518439
  8. flw is a key regulator of basal myosin oscillations and cell contractions underlying egg chamber elongation. PMID: 26888436
  9. Expression of myosin RLC variants that prevent or mimic phosphorylation both disrupt axis elongation, but have distinct effects at the molecular and cellular levels. PMID: 25071215
  10. A dual role of myosin II in regulating the development of the Drosophila wing, is reported. PMID: 23612302
  11. Study generated antibodies against the monophosphorylated (Sqh1P) and diphosphorylated (Sqh2P) forms of Sqh; in imaginal discs, Sqh1P predominantly localizes in the adherens junction, whereas Sqh2P locates to the apical domain. PMID: 20920606
  12. apical constriction and invagination of APC null tissue are independent of DE-cadherin elevation, but are dependent on Myosin II activity. PMID: 20102708
  13. the movement of myosin II to the contractile ring not only initiates cytokinesis but also completes the partitioning of cell fate determinants from the neuroblast to its daughter. PMID: 14667406
  14. Myosin II is specifically enriched in disassembling junctions, and its planar polarized localization and activity are required for planar junction remodelling and cell intercalation PMID: 15190355

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Database Links

KEGG: dme:Dmel_CG3595

STRING: 7227.FBpp0070842

UniGene: Dm.3

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