Recombinant Drosophila melanogaster POU domain protein CF1A (vvl)

Code CSB-YP322271DLU
MSDS
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Source Yeast
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Code CSB-EP322271DLU
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Source E.coli
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Code CSB-EP322271DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322271DLU
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Source Baculovirus
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Code CSB-MP322271DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
vvl
Uniprot No.
Alternative Names
vvl; Cf1a; CG10037POU domain protein CF1A; Chorion factor 1A; CF1-A; Protein drifter; Ventral veins lacking protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-427
Target Protein Sequence
MAATSYMTPP SGDLDMALGG GGYHTSSPRS AADAGEMKYM QHHHHHHAAA AAAAHHQLPS SPSPNGQGNG GGLGLGSGSG LGSWSALHPD PWMQTHHTHH LPAAAAVASA ADTVKQEMSH LSQQTRIQQG MASPHAAWHA PHAGHYAPTG GSPLQYHHAM NGMLHHPAHA VAAAHHQSVA PLHHTLRGES PQLHIHHHMG GGDRDAISGG EEDTPTSDDL EAFAKQFKQR RIKLGFTQAD VGLALGTLYG NVFSQTTICR FEALQLSFKN MCKLKPLLQK WLEEADSTTG SPTSIDKIAA QGRKRKKRTS IEVSVKGALE QHFHKQPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK RMTPPNTLGG DMMDGMPPGH MHHGGYHPHH DMHGSPMGTH SHSHSPPMLS PQNMQSSAVA AHQLAAH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to a DNA sequence element required for the expression of the dopa decarboxylase gene (Ddc) in specific dopaminergic neurons. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system.
Gene References into Functions
  1. Vvl is highly expressed in the prothoracic gland during embryogenesis and is enriched in the gland during larval development, suggesting that Vvl might function as a master transcriptional regulator in this tissue. PMID: 24945799
  2. Dfr/Vvl is highly expressed in a range of immunocompetent tissues, including the male ejaculatory duct, where its presence overlaps with and drives the expression of cecropin PMID: 20457811
  3. From lineage to wiring specificity. POU domain transcription factors control precise connections of Drosophila olfactory projection neurons. PMID: 12553905
  4. multiple roles for u-turn/ventral veinless in the development of the peripheral nervous system PMID: 12702660
  5. vvl acts downstream of the Hox gene lab and regulates specific aspects of neuronal differentiation within the tritocerebral neuromere during embryonic brain development PMID: 16326080

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Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-3 subfamily
Tissue Specificity
Coexpressed with acj6 in overlapping subsets of neurons in the embryonic epidermis and central nervous system. First detected in the precursor of the tracheal pits and the stomodeal invagination and later in the peripheral nervous system.
Database Links

KEGG: dme:Dmel_CG10037

STRING: 7227.FBpp0303092

UniGene: Dm.2556

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