Recombinant Drosophila melanogaster Protein Wnt-5 (Wnt5), partial

Code CSB-YP340985DLU
MSDS
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Source Yeast
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Code CSB-EP340985DLU
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Source E.coli
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Code CSB-EP340985DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340985DLU
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Source Baculovirus
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Code CSB-MP340985DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wnt5
Uniprot No.
Alternative Names
Wnt5; Wnt-3; WNT-5; CG6407Protein Wnt-5; dWnt-3; dWnt-5
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May have a role in limb and CNS development; may be a downstream target of Dll that acts in the specification of these primordia.
Gene References into Functions
  1. The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells. PMID: 27138857
  2. Wnt5 promotes dendrite termination on the periphery of sternites through the Ryk receptor family kinase Derailed (Drl) and the Rho GTPase guanine nucleotide exchange factor Trio in C4da neurons PMID: 26302791
  3. Wnt5 is required for projection neurons dendrites migration and targeting in the developing antennal lobe. PMID: 25378162
  4. WNT5 interacts with doughnut and derailed to mediate muscle attachment site selection PMID: 22403643
  5. Wnt5 is broadly expressed in the developing brain and regulates axonal development of mushroom body neurons. PMID: 21451033
  6. Derailed keeps axons out of the posterior commissure by acting as a receptor for Wnt5 PMID: 12660735
  7. Wnt5 protein is predominantly present on posterior commissural axons and at a low level on the anterior commissure and longitudinal projections PMID: 15282154
  8. WNT5 is required intrinsically within mushroom bodies neurons for proper axonal growth and probably interacts with the extrinsic DRL receptor in order to stop axonal growth. PMID: 17652353
  9. The wnt5 as an anterograde signal that is expressed by olfactory axons and demonstrate a previously unappreciated, yet powerful, role for glia in patterning the Drosophila olfactory map. PMID: 17934456
  10. Wnt5 regulates the growth of the Drosophila neuromuscular junction (NMJ) by signaling through the Derailed receptor. PMID: 17963254
  11. Src family kinases play novel roles in WNT5/Derailed-mediated signaling PMID: 18539923
  12. Derailed protein (Drl) and derailed protein-2 (Drl-2) play opposing roles in Wnt5 signaling during olfactory system development, depending upon the type of cells in which they are expressed. PMID: 19369566

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Wnt family
Tissue Specificity
Dynamic expression pattern during embryogenesis. Expression is seen in the limb primordia of the head and thoracic segments, mesodermal and neurogenic regions.
Database Links

KEGG: dme:Dmel_CG6407

STRING: 7227.FBpp0074394

UniGene: Dm.5375

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