Recombinant Drosophila melanogaster Protein giant (gt)

Code CSB-YP340119DLU
MSDS
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Source Yeast
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Code CSB-EP340119DLU
MSDS
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Source E.coli
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Code CSB-EP340119DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340119DLU
MSDS
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Source Baculovirus
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Code CSB-MP340119DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gt
Uniprot No.
Alternative Names
gt; CG7952; Protein giant
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-448
Target Protein Sequence
MLMHEKLMAG QFFDLKTDRK PLMHHHQYQH HQQQPLHHLP HSQLPVQGSL GLPKMDLYTA YAYQQQLLGA ALSQQQQQQQ QQQQHQQLQQ QHTSSAEVLD LSRRCDSVET PRKTPSPYQT SYSYGSGSPS ASPTSNLLYA AQMQQQQHQQ QQQQQQQQQQ LASLYPAFYY SNIKQEQATP TAAPPKVTPT ANLLQTFAAA SAAAAAAAAA SSTNSPRPAS NASTMQIDVL ENPLSPAVEA TTPTTSSSGE AGKNTRPFKA FPRDPLVIAA NFAATDVLLD NPRVERYTEY RKRVLEQIRS SNGGSRTVTN PKMRRTNSRS GSVNEGSSSN NNSESEDRAA AEESSDCDSQ AGNFESKSAT SSSSNLANAT AANSGISSGS QVKDAAYYER RRKNNAAAKK SRDRRRIKED EIAIRAAYLE RQNIELLCQI DALKVQLAAF TSAKVTTA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Represses the expression of both the krueppel and knirps segmentation gap genes. Binds, in vitro, to the krueppel regulatory elements CD1 and CD2. It is required in the early embryo for the development of portions of the head and abdomen.
Gene References into Functions
  1. there are two phases of transcriptional regulation for the gap gene giant in Drosophila PMID: 26806702
  2. two polycomb response elements-containing regions at the Polycomb-group proteins target gene, giant, one at the promoter, and another approximately 6 kb upstream. PMID: 24170735
  3. Results showed Gt repression roles in the regulation of two groups of partially overlapping stripes and that Gt morphogen activity is part of the mechanism responsible for the differential positioning of these stripes borders. PMID: 20925117
  4. gt likely controls ecdysone production indirectly by contributing the peptidergic phenotype of the PTTH-producing neurons in the embryo PMID: 20816678
  5. The Drosophila gap gene giant has an anterior segment identity function mediated through disconnected and teashirt. PMID: 18493063

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Subcellular Location
Nucleus.
Protein Families
BZIP family
Database Links

KEGG: dme:Dmel_CG7952

STRING: 7227.FBpp0070444

UniGene: Dm.5740

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