Recombinant Drosophila melanogaster Stress-activated protein kinase JNK (bsk)

Code CSB-YP310094DLU
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Source Yeast
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Code CSB-EP310094DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP310094DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bsk
Uniprot No.
Alternative Names
bsk; JNK; CG5680; Stress-activated protein kinase JNK; dJNK; EC 2.7.11.24; Protein basket
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-372
Target Protein Sequence
MTTAQHQHYT VEVGDTNFTI HSRYINLRPI GSGAQGIVCA AYDTITQQNV AIKKLSRPFQ NVTHAKRAYR EFKLMKLVNH KNIIGLLNAF TPQRNLEEFQ DVYLVMELMD ANLCQVIQMD LDHDRMSYLL YQMLCGIKHL HSAGIIHRDL KPSNIVVKAD CTLKILDFGL ARTAGTTFMM TPYVVTRYYR APEVILGMGY TENVDIWSVG CIMGEMIRGG VLFPGTDHID QWNKIIEQLG TPSPSFMQRL QPTVRNYVEN RPRYTGYSFD RLFPDGLFPN DNNQNSRRKA SDARNLLSKM LVIDPEQRIS VDEALKHEYI NVWYDAEEVD APAPEPYDHS VDEREHTVEQ WKELIYEEVM DYEAHNTNNR TR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Responds to activation by environmental stress by phosphorylating a number of transcription factors, primarily components of AP-1 such as Jra and also the transcriptional repressor aop, and thus regulates transcriptional activity. Component of the immune response activated by bacterial infection, and is involved in wound healing and in dorsal closure, a morphogenetic movement during embryogenesis. Functions in the systematic response to wounding acting downstream of the Hayan-phenoloxidase PPO1 cascade. Exhibits cytoprotective activity in neuronal cells in response to wounding to the integument. Controls the expression of a phosphatase, puckered, at the edges of wounded epidermal tissue and in the dorsal epithelium during dorsal closure. Regulates the activity of SREBP in neurons and thereby the accumulation of lipids in glia. Plays a role in postively regulating the expression of DIP2 independently of AP-1, thereby ensuring proper axon guidance in mushroom bodies.
Gene References into Functions
  1. Increased expression of the Drosophila JNK basket in the setting of reduced cindr expression was found to result in even more severe apoptosis, whilst ectopic death was found to be reduced if retinas were heterozygous for basket. PMID: 29133184
  2. n addition to significantly increasing the number of JNK target genes identified so far, our results reveal that the LE is a highly heterogeneous morphogenetic organizer, sculpted through crosstalk between JNK, segmental and AP signalling. This fine-tuning regulatory mechanism is essential to coordinate morphogenesis and dynamics of tissue sealing PMID: 28231245
  3. Diminished MTORC1-dependent JNK activation underlies the neurodevelopmental defects associated with lysosomal dysfunction. PMID: 26387958
  4. This study demonstrated that the mechanism by which Bsk is required for pruning is through reducing the membrane levels of the adhesion molecule Fasciclin II (FasII) PMID: 26586184
  5. Study solves the crystal structure of unphosphorylated DJNK in complex with adenylyl imidodiphosphate (AMP-PNP) and magnesium. PMID: 26377800
  6. In a genetic screen, we identified signaling by the EGFR pathway as important for apoptosis-induced proliferation acting downstream of JNK signaling PMID: 24497843
  7. We propose that JNK is a central stress response effector that is activated by nucleolar stress in Drosophila larvae PMID: 23412656
  8. dUev1a modulates TNF-JNK mediated tumor progression and cell death in Drosophila. PMID: 23726905
  9. Raw negatively regulates JNK signaling during gonad morphogenesis, and increased JNK signaling is sufficient to cause ensheathment defects. PMID: 22575490
  10. ectopic Dpp led to DJNK-dependent cardial apoptosis through the non-canonical TGF-beta pathway during late embryogenesis of Drosophila. PMID: 22114909
  11. stress-induced apoptosis in Drosophila is dependant on an amplification loop mediated by dp53 and JNK PMID: 21886179
  12. Pan-neuronal knockdown of JNK mRNA expression by RNA interference resulted in a decrease in both sleep and longevity. PMID: 22197814
  13. Data show that regeneration of genetically ablated tissue requires JNK activity, and that the JNK pathway is activated at the leading edges of healing tissue and not in apoptotic cells, and is required for regulation of healing and regenerative growth. PMID: 20215351
  14. These findings demonstrate that Eiger can initiate cell death through an IAP-sensitive cell death pathway via JNK signaling. PMID: 12065414
  15. Results introduce the Jun N-terminal kinase (JNK) signaling pathway as a genetic determinant of aging in Drosophila melanogaster. PMID: 14602080
  16. Relish limits the duration of JNK activation in response to Gram-negative infections. Degradation of TAK1 leads to a rapid termination of JNK signaling, resulting in a transient JNK-dependent response that precedes sustained induction of immune loci. PMID: 15037551
  17. The convergence of JNK signaling and IIS on Foxo provides a model to explain the effects of stress and nutrition on longevity. PMID: 15820683
  18. JNK acts as the downstream mediator of the LKB1-dependent apoptosis. PMID: 16273080
  19. Study found that the left-right asymmetric cell rearrangement in the circular visceral muscle was regulated by D-JNK signaling and required for the LR asymmetric looping of the anterior-midgut. PMID: 17915206
  20. regulators of JNK were identified; using genetic & phosphoproteomics data, an integrative network algorithm to construct a JNK phosphorylation network was implemented PMID: 18927396
  21. influences regeneration in the gut by directing proliferation of intestinal stem cells, contributes to the loss of tissue homeostasis in old and stressed intestines PMID: 18940735
  22. JNK activity upregulates genes involved in intracellular transport in order to provide an increased level of trafficking activity in cells undergoing complex morphogenetic arrangements such as dorsal closure. PMID: 19427848

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Tissue Specificity
During gastrulation, expression is seen in cells undergoing morphogenetic movements. By stage 9 of embryonic development, expression is ubiquitous. At stages 12-14, expression occurs in epidermis and central nervous system. At stage 15, expression is rest
Database Links

KEGG: dme:Dmel_CG5680

STRING: 7227.FBpp0079676

UniGene: Dm.1448

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