Recombinant Equine infectious anemia virus Protein Tat(tat)

Product Type Recombinant Protein
Uniprot No. P20920
Species Equine infectious anemia virus (strain Wyoming) (EIAV)
Gene Names tat
Protein Names Recommended name: Protein Tat Alternative name(s): E-Tat Transactivating regulatory protein eTat
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).
Subcellular Location Host nucleus, host nucleolus
Protein Families Lentiviruses Tat family

Product Details for Different Expression Systems

Product Name Recombinant Equine infectious anemia virus Protein Tat(tat),Yeast
Code CSB-YP321860EFZ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MADRRIPGTA EENLQKSSGG VPGQNTGGQE ARPNYHCQLC FLRSLGIDYL DASLRKKNKQ RLKAIQQGRQ PQYLL
Expression Region 1-75
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Equine infectious anemia virus Protein Tat(tat),E.coli
Code CSB-EP321860EFZ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MADRRIPGTA EENLQKSSGG VPGQNTGGQE ARPNYHCQLC FLRSLGIDYL DASLRKKNKQ RLKAIQQGRQ PQYLL
Expression Region 1-75
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Equine infectious anemia virus Protein Tat(tat),Baculovirus
Code CSB-BP321860EFZ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MADRRIPGTA EENLQKSSGG VPGQNTGGQE ARPNYHCQLC FLRSLGIDYL DASLRKKNKQ RLKAIQQGRQ PQYLL
Expression Region 1-75
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Equine infectious anemia virus Protein Tat(tat),Mammalian cell
Code CSB-MP321860EFZ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MADRRIPGTA EENLQKSSGG VPGQNTGGQE ARPNYHCQLC FLRSLGIDYL DASLRKKNKQ RLKAIQQGRQ PQYLL
Expression Region 1-75
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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